Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Ternes, Ana Paula Lausmann | - |
dc.contributor.author | Zemolin, Ana Paula | - |
dc.contributor.author | da Cruz, Litiele Cezar | - |
dc.contributor.author | da Silva, Gustavo Felipe | - |
dc.contributor.author | Saidelles, Ana Paula Fleig | - |
dc.contributor.author | de Paula, Mariane Trindade | - |
dc.contributor.author | Wagner, Caroline | - |
dc.contributor.author | Golombieski, Ronaldo Medeiros | - |
dc.contributor.author | Flores, Érico Marlon de Moraes | - |
dc.contributor.author | Picoloto, Rochele Sogari | - |
dc.contributor.author | Pereira, Antônio Batista | - |
dc.contributor.author | Franco, Jeferson Luis | - |
dc.contributor.author | Posser, Thaís | - |
dc.date.accessioned | 2015-04-20T12:59:41Z | - |
dc.date.available | 2015-04-20T12:59:41Z | - |
dc.date.issued | 2014-11-21 | - |
dc.identifier.issn | 1611-2156 | - |
dc.identifier.uri | http://hdl.handle.net/2003/34006 | - |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-7402 | - |
dc.description.abstract | Embryonic animals are especially susceptible to metal exposure. Manganese (Mn) is an es-sential element, but in excess it can induce toxicity. In this study we used Drosophila mela-nogaster as an embryonic model to investigate biochemical and behavioral alterations due to Mn exposure. Flies were treated with standard medium supplemented with MnCl2 at 0.1 mM, 0.5 mM or 1 mM from the egg to the adult stage. At 0.5 mM and 1 mM Mn, newly ecloded flies showed significantly enhanced locomotor activity when assessed by negative geotaxis behavior. In addition, a significant increase in Mn levels (p < 0.0001) was observed, while Ca, Fe, Cu, Zn and S levels were significantly decreased. A significant drop in cell viability occurred in flies exposed to 1 mM Mn. There was also an induction of reactive oxygen species at 0.5 mM and 1 mM Mn (p < 0.05). At 1 mM, Mn increased Catalase (p < 0.005), Superoxide Dismutase (p < 0.005) and Hsp83 (p < 0.0001) mRNA expression, without altering Catalase or Superoxide Dismutase activity; the activity of Thioredoxin reductase and Glutatione-S-transferase enzymes was increased. Mn treatment did not alter ERK or JNK1/2 phosphorylation, but at 1 mM caused an inhibition of p38MAPK phosphorylation. Together these data suggest mechanisms of adaptation in the fly response to Mn exposure in embryonic life. | en |
dc.language.iso | en | - |
dc.relation.ispartofseries | EXCLI Journal ; Vol. 13, 2014 | en |
dc.subject | Manganese | en |
dc.subject | Drosophila melanogaster | en |
dc.subject | MAPK | en |
dc.subject | oxidative stress | en |
dc.subject | Thioredoxin reductase | en |
dc.subject.ddc | 610 | - |
dc.title | Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure | en |
dc.type | Text | - |
dc.type.publicationtype | article | - |
dcterms.accessRights | open access | - |
eldorado.dnb.zdberstkatid | 2132560-1 | - |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Posser_20112014_proof.pdf | DNB | 788.22 kB | Adobe PDF | View/Open |
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