Autor(en): | Neumann, Heinz Neumann-Staubitz, Petra Witte, Anna Summerer, Daniel |
Titel: | Epigenetic chromatin modification by amber suppression technology |
Sprache (ISO): | en |
Zusammenfassung: | The genetic incorporation of unnatural amino acids (UAAs) into proteins by amber suppression technology provides unique avenues to study protein structure, function and interactions both in vitro and in living cells and organisms. This approach has been particularly useful for studying mechanisms of epigenetic chromatin regulation, since these extensively involve dynamic changes in structure, complex formation and posttranslational modifications that are difficult to access by traditional approaches. Here, we review recent achievements in this field, emphasizing UAAs that help to unravel protein-protein interactions in cells by photo-crosslinking or that allow the biosynthesis of proteins with defined posttranslational modifications for studying their function and turnover in vitro and in cells. |
URI: | http://hdl.handle.net/2003/38114 http://dx.doi.org/10.17877/DE290R-20095 |
Erscheinungsdatum: | 2018-08 |
Enthalten in den Sammlungen: | Lehrstuhl für Chemische Biologie der Nukleinsäuren |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
CurrOp_2018.pdf | 4.12 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource ist urheberrechtlich geschützt. |
Diese Ressource ist urheberrechtlich geschützt.
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