Authors: | Rolf, Jascha Julsing, Mattijs K. Rosenthal, Katrin Lütz, Stephan |
Title: | A gram-scale limonene production process with engineered escherichia coli |
Language (ISO): | en |
Abstract: | Monoterpenes, such as the cyclic terpene limonene, are valuable and important natural products widely used in food, cosmetics, household chemicals, and pharmaceutical applications. The biotechnological production of limonene with microorganisms may complement traditional plant extraction methods. For this purpose, the bioprocess needs to be stable and ought to show high titers and space-time yields. In this study, a limonene production process was developed with metabolically engineered Escherichia coli at the bioreactor scale. Therefore, fed-batch fermentations in minimal medium and in the presence of a non-toxic organic phase were carried out with E. coli BL21 (DE3) pJBEI-6410 harboring the optimized genes for the mevalonate pathway and the limonene synthase from Mentha spicata on a single plasmid. The feasibility of glycerol as the sole carbon source for cell growth and limonene synthesis was examined, and it was applied in an optimized fermentation setup. Titers on a gram-scale of up to 7.3 g·Lorg−1 (corresponding to 3.6 g·L−1 in the aqueous production phase) were achieved with industrially viable space-time yields of 0.15 g·L−1·h−1. These are the highest monoterpene concentrations obtained with a microorganism to date, and these findings provide the basis for the development of an economic and industrially relevant bioprocess. |
Subject Headings: | Monoterpenes Limonene Glycerol Mevalonate pathway Reaction engineering Bioprocess Biocatalyst Two-liquid phase fermentation In situ product removal |
URI: | http://hdl.handle.net/2003/39092 http://dx.doi.org/10.17877/DE290R-21010 |
Issue Date: | 2020-04-18 |
Rights link: | https://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | Arbeitsgruppe Bioverfahrenstechnik |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
molecules-25-01881-v3.pdf | 1.29 MB | Adobe PDF | View/Open |
This item is protected by original copyright |
This item is licensed under a Creative Commons License