Authors: Suter, Dieter
Bingham, Stephen J.
Börger, Birgit
Thomson, Andrew J.
Gutschank, Jörg
Title: Probing the electronic structure of transition metal ion centres in proteins by coherent Raman detected electron paramagnetic resonance spectroscopy
Language (ISO): en
Abstract: The simultaneous excitation of a paramagnetic sample with optical (laser) and microwave radiation can cause an amplitude or phase modulation of the transmitted light at the microwave frequency. The detection of this modulation indicates the presence of coupled optical and electron paramagnetic resonance (EPR) transitions in the sample. Here we report the first application of this technique to a biomolecule: the blue copper centre of Pseudomonas aeruginosa azurin. Using optical excitation at 686 nm, in the thiol to copper(II) charge transfer band, we measure a coherent Raman-detected EPR spectrum of a frozen aqueous solution. Its lineshape is characteristic of the magnetic circular dichroism along each principal g-value axis. This information allows electronic and structural models of transition metal ion centres in proteins to be tested.
URI: http://hdl.handle.net/2003/25352
http://dx.doi.org/10.17877/DE290R-2687
Publishers Link: http://e3.physik.tu-dortmund.de/~suter/eprints/Raman-EPR_of_metal_proteins.pdf
Issue Date: 2000-02
Rights: © 2000 Springer
Publisher: Springer
URL: http://dx.doi.org/10.1007/s007750050004
Citation: Bingham, Stephen J.; Börger, Birgit; Gutschank, Jörg; Suter, Dieter, Thomson, Andrew J.: Probing the electronic structure of transition metal ion centres in proteins by coherent Raman detected electron paramagnetic resonance spectroscopy. In: Journal of Biological Inorganic Chemistry Nr. 1, Jg. 5(2000), S. 30-35.
Appears in Collections:Suter, Dieter Prof. Dr.

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