Authors: | Choi, Yook-Wah Fielding, Burtram Goh, Phuay-Yee Hong, Wanjin Lim, Seng Gee Ooi, Eng-Eong Shuo, Shen Tan, Timothy H. P. Tan, Yee-Joo |
Title: | Cellular Characterization of SARS Coronavirus Nucleocapsid |
Language (ISO): | en |
Abstract: | The Severe and Acute Respiratory Syndrome coronavirus (SARS CoV) is a newly-emerged virus that caused an outbreak of atypical pneumonia in the winter of 2002-2003. Polyclonal antibodies raised against the nucleocapsid (N) of the SARS CoV showed the localization of N to the cytoplasm and the nucleolus in virus-infected and N-expressing Vero E6 cells. Like other coronavirus N proteins, the SARS N is probably a phosphoprotein. N protein expressed in mammalian cells is apparently able to "spread" to neighboring cells. For N to spread to neighboring cells, it must be exported out of the expressing cells. This is shown by the immunoprecipitation of N from the culture medium of a stable cell line expressing myc-N. Deletion studies showed that the 27 kD C-terminal domain of N (C1/2) is the minimal region of N that can spread to other cells. The nucleolar localization and spreading of N are artefacts of fixation, reminiscent of other protein-transduction domain (PTD)-containing proteins. |
Subject Headings: | coronavirus fixation nucleocapsid nucleolus SARS transduction |
URI: | http://hdl.handle.net/2003/25640 http://dx.doi.org/10.17877/DE290R-14553 |
Issue Date: | 2004-11-01 |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Goh10-04proofrev.pdf | DNB | 541.27 kB | Adobe PDF | View/Open |
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