Autor(en): | Cui, Hualei Li, Yan Lu, Huimin Tian, Qi |
Titel: | LY294002 induces differentiation and inhibits invasion of glioblastoma cells by targeting GSK-3β and MMP |
Sprache (ISO): | en |
Zusammenfassung: | Glioblastomas are the most common and devastating primary tumors of the central nervous system, with high proliferative capacity, aggressive invasion, and resistance to conventional therapies. Differentiation therapy has emerged as a promising candidate modality. Here we show that the traditional phosphatidylinositol 3 kinase (PI3K) inhibitor LY294002 is capable of inducing differentiation of C6 glioblastoma cells characterized by morphological changes to astrocytic phenotype, increase in differentiation marker protein glial fibrillary acidic pro-tein and inhibition of proliferation. Small interfering RNA against glycogen synthase kinase-3β (GSK-3β) suppresses the induced-differentiation and invasiveness in C6 cells. LY294002 also inhibits MMP-9 expression and invasion of C6 cells, assembling the role of metalloprotease (MMP) inhibitor AG3340. Taken together, these findings suggest differentiation-inducing and invasion-inhibitory effectiveness of LY294002 in glioblastomas, most likely involving inhibition of GSK-3β and MMP respectively. |
Schlagwörter: | differentiation glioblastoma GSK-3β invasion LY294002 MMP |
URI: | http://hdl.handle.net/2003/29626 http://dx.doi.org/10.17877/DE290R-4914 |
Erscheinungsdatum: | 2012-09-19 |
Enthalten in den Sammlungen: | Original Articles |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
QiTian_27022012_proof.pdf | DNB | 443.18 kB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource ist urheberrechtlich geschützt. |
Diese Ressource ist urheberrechtlich geschützt. rightsstatements.org