Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Kil-Nam | - |
dc.contributor.author | Yang, Hye-Won | - |
dc.contributor.author | Ko, Seok-Chun | - |
dc.contributor.author | Ko, Yeong-Jong | - |
dc.contributor.author | Kim, Eun-A | - |
dc.contributor.author | Roh, Seong Woon | - |
dc.contributor.author | Ko, Eun-Yi | - |
dc.contributor.author | Ahn, Ginnae | - |
dc.contributor.author | Heo, Soo-Jin | - |
dc.contributor.author | Jeon, You-Jin | - |
dc.contributor.author | Yoon, Weon-Jong | - |
dc.contributor.author | Hyun, Chang-Gu | - |
dc.contributor.author | Kim, Daekyung | - |
dc.date.accessioned | 2014-11-25T13:19:49Z | - |
dc.date.available | 2014-11-25T13:19:49Z | - |
dc.date.issued | 2014-07-21 | - |
dc.identifier.issn | 1611-2156 | - |
dc.identifier.uri | http://hdl.handle.net/2003/33712 | - |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-6945 | - |
dc.description.abstract | In this study, we investigated the ability of 6,7-dimethoxy-4-methylcoumarin (DMC) to inhibit lipopolysaccharide (LPS)-induced expression of pro-inflammatory mediators in mouse macrophage (RAW 264.7) cells, and the molecular mechanism through which this inhibition occurred. Our results indicated that DMC down regulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, thereby reducing the production of NO and prostaglandin E2 (PGE2) in LPS-activated RAW 264.7 cells. Furthermore, DMC suppressed LPS-induced production of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. To elucidate the mechanism underlying the anti- inflammatory activity of DMC, we assessed its effects on the mitogen-activated protein kinase (MAPK) pathway and the activity and expression of nuclear transcription factor kappa-B (NF-κB). The experiments demonstrated that DMC inhibited LPS-induced phosphorylation of extracellular signal-regulated kinases (ERKs), c-Jun N-terminal kinase (JNK), and p38. In addition, it attenuated LPS-induced NF-κB activation via the inhibition of IκB-α phosphorylation. Taken together, these data suggest that DMC exerts its anti-inflammatory effects in RAW 264.7 cells through the inhibition of LPS-stimulated NF-κB and MAPK signaling, thereby downregulating the expression of pro-inflammatory mediators. | en |
dc.language.iso | en | - |
dc.relation.ispartofseries | EXCLI Journal ; Vol. 13, 2014 | en |
dc.subject | 6,7-Dimethoxy-4-methylcoumarin (DMC) | en |
dc.subject | anti-inflammatory | en |
dc.subject | RAW 264.7 cells | en |
dc.subject | NF-κB | en |
dc.subject | MAPKs | en |
dc.subject.ddc | 610 | - |
dc.title | 6,7-Dimethoxy-4-methylcoumarin suppresses pro-inflammatory mediator expression through inactivation of the NF-kappaB and MAPK pathways in LPS-induced RAW 264.7 cells | en |
dc.type | Text | - |
dc.type.publicationtype | article | - |
dcterms.accessRights | open access | - |
eldorado.dnb.zdberstkatid | 2132560-1 | - |
Appears in Collections: | Original Articles |
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