Authors: Ternes, Ana Paula Lausmann
Zemolin, Ana Paula
da Cruz, Litiele Cezar
da Silva, Gustavo Felipe
Saidelles, Ana Paula Fleig
de Paula, Mariane Trindade
Wagner, Caroline
Golombieski, Ronaldo Medeiros
Flores, Érico Marlon de Moraes
Picoloto, Rochele Sogari
Pereira, Antônio Batista
Franco, Jeferson Luis
Posser, Thaís
Title: Drosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposure
Language (ISO): en
Abstract: Embryonic animals are especially susceptible to metal exposure. Manganese (Mn) is an es-sential element, but in excess it can induce toxicity. In this study we used Drosophila mela-nogaster as an embryonic model to investigate biochemical and behavioral alterations due to Mn exposure. Flies were treated with standard medium supplemented with MnCl2 at 0.1 mM, 0.5 mM or 1 mM from the egg to the adult stage. At 0.5 mM and 1 mM Mn, newly ecloded flies showed significantly enhanced locomotor activity when assessed by negative geotaxis behavior. In addition, a significant increase in Mn levels (p < 0.0001) was observed, while Ca, Fe, Cu, Zn and S levels were significantly decreased. A significant drop in cell viability occurred in flies exposed to 1 mM Mn. There was also an induction of reactive oxygen species at 0.5 mM and 1 mM Mn (p < 0.05). At 1 mM, Mn increased Catalase (p < 0.005), Superoxide Dismutase (p < 0.005) and Hsp83 (p < 0.0001) mRNA expression, without altering Catalase or Superoxide Dismutase activity; the activity of Thioredoxin reductase and Glutatione-S-transferase enzymes was increased. Mn treatment did not alter ERK or JNK1/2 phosphorylation, but at 1 mM caused an inhibition of p38MAPK phosphorylation. Together these data suggest mechanisms of adaptation in the fly response to Mn exposure in embryonic life.
Subject Headings: Manganese
Drosophila melanogaster
oxidative stress
Thioredoxin reductase
Issue Date: 2014-11-21
Appears in Collections:Original Articles

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