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dc.contributor.authorTernes, Ana Paula Lausmann-
dc.contributor.authorZemolin, Ana Paula-
dc.contributor.authorda Cruz, Litiele Cezar-
dc.contributor.authorda Silva, Gustavo Felipe-
dc.contributor.authorSaidelles, Ana Paula Fleig-
dc.contributor.authorde Paula, Mariane Trindade-
dc.contributor.authorWagner, Caroline-
dc.contributor.authorGolombieski, Ronaldo Medeiros-
dc.contributor.authorFlores, Érico Marlon de Moraes-
dc.contributor.authorPicoloto, Rochele Sogari-
dc.contributor.authorPereira, Antônio Batista-
dc.contributor.authorFranco, Jeferson Luis-
dc.contributor.authorPosser, Thaís-
dc.date.accessioned2015-04-20T12:59:41Z-
dc.date.available2015-04-20T12:59:41Z-
dc.date.issued2014-11-21-
dc.identifier.issn1611-2156-
dc.identifier.urihttp://hdl.handle.net/2003/34006-
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-7402-
dc.description.abstractEmbryonic animals are especially susceptible to metal exposure. Manganese (Mn) is an es-sential element, but in excess it can induce toxicity. In this study we used Drosophila mela-nogaster as an embryonic model to investigate biochemical and behavioral alterations due to Mn exposure. Flies were treated with standard medium supplemented with MnCl2 at 0.1 mM, 0.5 mM or 1 mM from the egg to the adult stage. At 0.5 mM and 1 mM Mn, newly ecloded flies showed significantly enhanced locomotor activity when assessed by negative geotaxis behavior. In addition, a significant increase in Mn levels (p < 0.0001) was observed, while Ca, Fe, Cu, Zn and S levels were significantly decreased. A significant drop in cell viability occurred in flies exposed to 1 mM Mn. There was also an induction of reactive oxygen species at 0.5 mM and 1 mM Mn (p < 0.05). At 1 mM, Mn increased Catalase (p < 0.005), Superoxide Dismutase (p < 0.005) and Hsp83 (p < 0.0001) mRNA expression, without altering Catalase or Superoxide Dismutase activity; the activity of Thioredoxin reductase and Glutatione-S-transferase enzymes was increased. Mn treatment did not alter ERK or JNK1/2 phosphorylation, but at 1 mM caused an inhibition of p38MAPK phosphorylation. Together these data suggest mechanisms of adaptation in the fly response to Mn exposure in embryonic life.en
dc.language.isoen-
dc.relation.ispartofseriesEXCLI Journal ; Vol. 13, 2014en
dc.subjectManganeseen
dc.subjectDrosophila melanogasteren
dc.subjectMAPKen
dc.subjectoxidative stressen
dc.subjectThioredoxin reductaseen
dc.subject.ddc610-
dc.titleDrosophila melanogaster - an embryonic model for studying behavioral and biochemical effects of manganese exposureen
dc.typeText-
dc.type.publicationtypearticle-
dcterms.accessRightsopen access-
eldorado.dnb.zdberstkatid2132560-1-
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