Authors: Ahna, Ginnae
Lee, WonWoo
Kim, Kil-Nam
Lee, Ji-Hyeok
Heo, Soo-Jin
Kang, Nalae
Lee, Seung-Hong
Ahnf, Chang-Bum
Jeon, You-Jin
Title: A sulfated polysaccharide of Ecklonia cava inhibits the growth of colon cancer cells by inducing apoptosis
Language (ISO): en
Abstract: We investigated anticancer effects of the crude polysaccharides (CPs) isolated from Ecklonia cava enzymatic extracts using AMG, Viscozyme, Protamex, and Alcalase enzyme against a colon cancer cell line, CT26 cells. Among them, the CP of Protamex extract (PCP) contained the highest fucose and sulfated group contents and showed the highest growth inhibitory effect against CT-26 cells. In addition, PCP dose-dependently increased the formation of apoptotic body and the percentage of Sub-G1 DNA contents. Also, PCP activated caspase 9 and PARP as regulating the expressions of Bax and Bcl-2. Moreover, PPP2, a fraction purified from PCP showed the highest growth inhibitory effect against CT 26 cells with the increased fucose and sulfated group contents. The results demonstrate that the isolated SP containing plentiful fucose and sulfated group contents has the anticancer effect on colon cancer cells via regulation of Bcl-2/Bax signal pathway.
Subject Headings: Ecklonia cava
sulfated polysaccharides
enzyme-assisted extraction
murine colon cancer cells (CT-26 cells)
Issue Date: 2015-02-24
Appears in Collections:Original Articles

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