Authors: | Ko, Eun-Yi Lee, Seung-Hong Ko, Ji-Yeon Moon, Jeong Yong Yoon, Weon-Jong Ahn, Ginnae Roh, Seong Woon Cho, Kichul Jeon, You-Jin Kim, Daekyung Kim, Kil-Nam |
Title: | Trans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cells |
Language (ISO): | en |
Abstract: | The anticancer effects of trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP), a chalcone derivative, were investigated in human leukemia HL-60 cells. Treatment of HL-60 cells with various concentration of DPEP resulted in a sequence of events characteristic of apoptosis, including loss of cell viability, morphological changes, and increased sub-G1 DNA content. We demonstrated that DPEP elevates reactive oxygen species (ROS) levels in HL-60 cells, and that the ROS scavenger N-acetylcysteine (NAC) could block DPEP-induced ROS generation and apoptosis. Western blot analysis revealed that DPEP inhibits Bcl-xL expression, leading to caspase-3 activation and poly-ADP-ribose polymerase (PARP) cleavage, thereby inducing apoptosis. However, NAC pretreatment significantly inhibited the activation of caspase-3 and PARP cleavage and reduced Bcl-xL levels. These findings provide the first evidence that DPEP may inhibit the growth of HL-60 cells and induce apoptosis through a ROS-mediated Bcl-xL pathway. |
Subject Headings: | anticancer trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP) apoptosis reactive oxygen species (ROS) Bcl-xL |
URI: | http://hdl.handle.net/2003/35025 http://dx.doi.org/10.17877/DE290R-17073 |
Issue Date: | 2015-08-03 |
Rights link: | http://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | Original Articles |
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Ko_Kim_03082015_proof.pdf | DNB | 850.64 kB | Adobe PDF | View/Open |
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