Authors: | Ko, Eun-Yi Yoon, Weon-Jong Lee, Hae-Won Heo, Soo-Jin Ko, Young-Hwan Fernando, I.P. Shanura Cho, Kichul Lee, Chi-Heon Hur, Sung-Pyo Cho, Su-Hyeon Ahn, Ginnae Kim, Daekyung Kim, Kil-Nam |
Title: | Anti-inflammatory effect of supercritical extract and its constituents from Ishige okamurae |
Language (ISO): | en |
Abstract: | The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it’s anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced IκB-α degradation and p50 NF-κB activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPS-activated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-κB. |
Subject Headings: | supercritical fluid extraction linoleic acid Ishige okamurae anti-inflammatory NF- κB |
URI: | http://hdl.handle.net/2003/35618 http://dx.doi.org/10.17877/DE290R-17659 |
Issue Date: | 2016-06-28 |
Rights link: | https://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Kim_Kim_28062016_proof.pdf | DNB | 1.06 MB | Adobe PDF | View/Open |
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