Authors: Ko, Eun-Yi
Yoon, Weon-Jong
Lee, Hae-Won
Heo, Soo-Jin
Ko, Young-Hwan
Fernando, I.P. Shanura
Cho, Kichul
Lee, Chi-Heon
Hur, Sung-Pyo
Cho, Su-Hyeon
Ahn, Ginnae
Kim, Daekyung
Kim, Kil-Nam
Title: Anti-inflammatory effect of supercritical extract and its constituents from Ishige okamurae
Language (ISO): en
Abstract: The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it’s anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced IκB-α degradation and p50 NF-κB activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPS-activated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-κB.
Subject Headings: supercritical fluid extraction
linoleic acid
Ishige okamurae
NF- κB
Issue Date: 2016-06-28
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