Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Nabu, Sunanta | - |
dc.contributor.author | Lawung, Ratana | - |
dc.contributor.author | Isarankura-Na-Ayudhya, Patcharee | - |
dc.contributor.author | Roytrakul, Sittiruk | - |
dc.contributor.author | Dolprasit, Supamas | - |
dc.contributor.author | Sengyee, Sineenart | - |
dc.contributor.author | Isarankura-Na-Ayudhya, Chartchalerm | - |
dc.contributor.author | Prachayasittikul, Virapong | - |
dc.date.accessioned | 2018-02-05T12:45:22Z | - |
dc.date.available | 2018-02-05T12:45:22Z | - |
dc.date.issued | 2017-11-08 | - |
dc.identifier.issn | 1611-2156 | - |
dc.identifier.uri | http://hdl.handle.net/2003/36356 | - |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-18357 | - |
dc.description.abstract | Neisseria gonorrhoeae strains displaying reduced susceptibility and resistance to extended-spectrum cephalo- sporins (ESCs) are major public health concerns. Although resistance mechanisms of ESCs have extensively been studied, the proteome-wide investigation on the biological response to the antibiotic stress is still limited. Herein, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS analy- sis was applied to investigate the global protein expression under ESC stresses of ESC-susceptible and ESC- reduced susceptible N. gonorrhoeae strains. Upon exposure to ceftriaxone, 14 and 21 proteins of ESC- susceptible and ESC-reduced susceptible strains, respectively, were shown to be differentially expressed. In the meanwhile, differential expressions of 13 and 17 proteins were detected under cefixime stress for ESC- susceptible and ESC-reduced susceptible strains, respectively. ESC antibiotics have been proven to trigger the expression of several proteins implicated in a variety of biological functions including transport system, energy metabolism, stress response and pathogenic virulence factors. Interestingly, macrophage infectivity potentiators (Ng-MIP) showed increased expression for ESC-reduced susceptible strain under ESC stress. The altered ex- pression of Ng-MIP was found to be a unique response to ESC stresses. Our finding proposes a broad view on proteomic changes in N. gonorrhoeae in response to ESC antibiotics that provides further insights into the gono- coccal antimicrobial resistance and physiological adaptation mechanism. | en |
dc.language.iso | en | - |
dc.relation.ispartofseries | EXCLI Journal;Vol. 16 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Neisseria gonorrhoeae | en |
dc.subject | Extended-spectrum cephalosporins | en |
dc.subject | Antimicrobial resistance | en |
dc.subject | Proteomics | en |
dc.subject.ddc | 610 | - |
dc.title | Comparative proteomics analysis of Neisseria gonorrhoeae strains in response to extended-spectrum cephalosporins | en |
dc.type | Text | - |
dc.type.publicationtype | article | - |
dcterms.accessRights | open access | - |
eldorado.dnb.zdberstkatid | 2132560-1 | - |
eldorado.secondarypublication | true | - |
Appears in Collections: | Original Articles |
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File | Description | Size | Format | |
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Isarankura-Na-Ayudhya_08112017_proof.pdf | DNB | 4.32 MB | Adobe PDF | View/Open |
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