Authors: | Mohammadi-Farsani, Azadeh Habibi-Roudkenar, Mehryar Golkar, Majid Shokrgozar, Mohammad Ali Jahanian-Najafabadi, Ali KhanAhmad, Hossein Valiyari, Samira Bouzari, Saeid |
Title: | A-NGR fusion protein induces apoptosis in human cancer cells |
Language (ISO): | en |
Abstract: | The NGR peptide is one of the well-known peptides for targeting tumor cells. It has the ability to target aminopeptidase N (CD13) on tumor cells or the tumor vascular endothelium. In this study, the NGR peptide was used for targeting A subunit of the Shiga toxin to cancer cells. The cytotoxic effect of the A-NGR fusion protein was assessed on HT1080, U937, HT29 cancer cells and MRC-5 normal cells. For this purpose, cells were treated with different concentrations of A-NGR (0.5-40 μg/ml). The evaluation of cell viability was achieved by MTT assay. Apoptosis was determined by annexin-V/PI double staining flow cytometry. Alterations in the mRNA expression of apoptosis - related genes were assessed by real time RT- PCR. The results showed that A-NGR fusion protein effectively inhibited the growth of HT1080 and U937 cancer cells in comparison to negative control (PBS) but for CD13-negative HT-29 cancer cells, only at high concentrations of fusion protein was inhibited growth recorded. On the other hand, A-NGR had little cytotoxic effect on MRC-5 normal cells. The flow cytometry results showed that A-NGR induces apoptosis. Furthermore, the results of real time RT-PCR revealed that A-NGR significantly increases the mRNA expression of caspase 3 and caspase 9. Conclusively, A-NGR fusion protein has the ability of targeting CD13-positive cancer cells, the cytotoxic effect on CD13-positive cancer cells as well as has low cytotoxic effect on normal cells. |
Subject Headings: | Shiga toxin NGR peptide Apoptosis Cytotoxicity |
URI: | http://hdl.handle.net/2003/37647 http://dx.doi.org/10.17877/DE290R-19642 |
Issue Date: | 2018-06-25 |
Rights link: | https://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Bouzari_25062018_proof.pdf | DNB | 181.64 kB | Adobe PDF | View/Open |
Bouzari_25062018_supplementary_data.pdf | DNB | 60.25 kB | Adobe PDF | View/Open |
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