Authors: | Yousefi, Forouzan Lavi Arab, Fahimeh Jaafari, Mahmoud Reza Rastin, Maryam Tabasi, Nafiseh Hatamipour, Mahdi Nikkhah, Karim Mahmoudi, Mahmoud |
Title: | Immunoregulatory, proliferative and anti-oxidant effects of nanocurcuminoids on adipose-derived mesenchymal stem cells |
Language (ISO): | en |
Abstract: | Curcuminoids are dietary complexes extracted from the seeds of Curcuma longa L. that contain curcumin, bisdemethoxycurcumin and desmethoxycurcumin. Curcuminoids are popular for their pleiotropic therapeutic functions, such as their anti-inflammatory and anti-oxidant effects. Nonetheless, their clinical use is associated with poor systemic bioavailability and insolubility. The nano-formulation of curcuminoids eliminates these shortcomings. In the present study, we explored immunoregulatory, proliferative and anti-oxidant effects of nanocurcuminoids on adipose-derived mesenchymal stem cells (AT-MSCs). Flow cytometry analysis and MTT assay were employed to explore the effects of nanocurcuminoids on the apoptosis and proliferation of adipose-derived MSCs (AT-MSCs). The anti-oxidant effect of nanocurcuminoids on AT-MSCs also was examined. The immune regulatory effect of nanocurcuminoids was evaluated by the flow cytometric measurement of the T regulatory (Treg) population. The expression of inflammatory and anti-inflammatory cytokines was quantified using real-time PCR. Our findings demonstrate that low concentrations of nanocurcuminoids are beneficial for MSC proliferation, protection of MSCs from apoptosis, reducing inflammatory cytokines and SOD activity. A high concentration of nanocurcuminoids increases the population of Tregs and elevates the expression of TGFβ and FOXP3 genes. The beneficial effects of nanocurcuminoids on AT-MSCs were mainly observed at low doses of nanocurcuminoids. |
Subject Headings: | Immunoregulatory Antioxidant Nanocurcuminoids Adipose-derived mesenchymal stem cells |
URI: | http://hdl.handle.net/2003/38564 http://dx.doi.org/10.17877/DE290R-20483 |
Issue Date: | 2019-06-17 |
Rights link: | https://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Mahmoudi_17062019_proof.pdf | DNB | 853.55 kB | Adobe PDF | View/Open |
Mahmoudi_17062019_supplementary_data.pdf | DNB | 246.41 kB | Adobe PDF | View/Open |
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