Authors: Li, Pan
Ren, Kai
Liang, Yin yin
Liu, Ji kai
Liang, Zhuo wen
Zhang, Yong feng
Title: Aloin promotes cell apoptosis by targeting HMGB1-TLR4-ERK axis in human melanoma cells
Language (ISO): en
Abstract: Aloin (ALO) is the major anthraquinone glycoside purified from the Aloe species. It is well known for its anti-tumor effects. However, the protective effects of ALO in melanoma cancer and underlying molecular mechanism remain unclear. High-mobility group protein B1 (HMGB1) is an intracellular protein, which has closely association with cell survival, proliferation and metastasis in various cancers. In this study, we explored the effect of ALO on cell survival and apoptosis by targeting HMGB1 signal pathway. We confirmed that ALO exerts a strong effect on promoting cell apoptosis of melanoma cells in vitro. Furthermore, HMGB1 release was significantly inhibited in melanoma cancer cells treated with ALO. Knockdown of HMGB1 could enhance melanoma cell death that is induced by ALO treatment. Moreover, HMGB1 facilitated ALO mediated melanoma cell apoptosis by binding to its receptor, Toll-like receptor 4 and activating extracellular regulated protein kinases (ERK) signal pathway. Altogether, our study demonstrated that ALO plays an important role in promoting apoptosis of melanoma cells by inhibiting HMGB1 release and activation of downstream ERK signal pathway.
Subject Headings: Aloin
Melanoma
Apoptosis
HMGB1
ERK signal pathway
URI: http://hdl.handle.net/2003/39853
http://dx.doi.org/10.17877/DE290R-21744
Issue Date: 2020-05-14
Provenance: IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
Citation: https://www.excli.de/index.php/excli/article/view/1951
Appears in Collections:Original Articles 2020

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