Autor(en): Thomas, Fabian
Schmidt, Christina
Kayser, Oliver
Titel: Bioengineering studies and pathway modeling of the heterologous biosynthesis of tetrahydrocannabinolic acid in yeast
Sprache (ISO): en
Zusammenfassung: Heterologous biosynthesis of tetrahydrocannabinolic acid (THCA) in yeast is a biotechnological process in Natural Product Biotechnology that was recently introduced. Based on heterologous genes from Cannabis sativa and Streptomyces spp. cloned into Saccharomyces cerevisiae, the heterologous biosynthesis was fully embedded as a proof of concept. Low titer and insufficient biocatalytic rate of most enzymes require systematic optimization of recombinant catalyst by protein engineering and consequent C-flux improvement of the yeast chassis for sufficient precursor (acetyl-CoA), energy (ATP), and NADH delivery. In this review basic principles of in silico analysis of anabolic pathways towards olivetolic acid (OA) and cannabigerolic acid (CBGA) are elucidated and discussed to identify metabolic bottlenecks. Based on own experimental results, yeasts are discussed as potential platform organisms to be introduced as potential cannabinoid biofactories. Especially feeding strategies and limitations in the committed mevalonate and olivetolic acid pathways are in focus of in silico and experimental studies to validate the scientific and commercial potential as a realistic alternative to the plant Cannabis sativa.
Schlagwörter: Cannabis sativa
Synthetic biology
Natural Product Biotechnology
Erscheinungsdatum: 2020-10-12
Rechte (Link):
Enthalten in den Sammlungen:Lehrstuhl Technische Biochemie

Dateien zu dieser Ressource:
Datei Beschreibung GrößeFormat 
Thomas2020_Article_BioengineeringStudiesAndPathwa.pdf669.29 kBAdobe PDFÖffnen/Anzeigen

Diese Ressource ist urheberrechtlich geschützt.

Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons