Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Mukherjee, Sanjib K. | - |
dc.contributor.author | Knop, Jim-Marcel | - |
dc.contributor.author | Möbitz, Simone | - |
dc.contributor.author | Winter, Roland | - |
dc.date.accessioned | 2021-07-29T07:54:22Z | - |
dc.date.available | 2021-07-29T07:54:22Z | - |
dc.date.issued | 2020-05-26 | - |
dc.identifier.uri | http://hdl.handle.net/2003/40342 | - |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-22217 | - |
dc.description.abstract | The effect of an amyloidogenic intrinsically disordered protein, α-synuclein, which is associated with Parkinson's disease (PD), on the conformational dynamics of a DNA hairpin (DNA-HP) was studied by employing the single-molecule Förster resonance energy transfer method. The open-to-closed conformational equilibrium of the DNA-HP is drastically affected by binding of monomeric α-synuclein to the loop region of the DNA-HP. Formation of a protein-bound intermediate conformation is fostered in the presence of an aqueous two-phase system mimicking intracellular liquid-liquid phase separation. Using pressure modulation, additional mechanistic information about the binding complex could be retrieved. Hence, in addition to toxic amyloid formation, α-synuclein may alter expression profiles of disease-modifying genes in PD. Furthermore, these findings might also have significant bearings on the understanding of the physiology of organisms thriving at high pressures in the deep sea. | en |
dc.language.iso | en | de |
dc.relation.ispartofseries | Chemistry - a european journal;Vol. 26. 2020, issue 48, pp 10987-10991 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Biophysics | en |
dc.subject | DNA hairpin | en |
dc.subject | High pressure chemistry | en |
dc.subject | smFRET | en |
dc.subject | a-synuclein | en |
dc.subject.ddc | 540 | - |
dc.title | Alteration of the conformational dynamics of a DNA hairpin by α-synuclein in the presence of aqueous two-phase systems | en |
dc.type | Text | de |
dc.type.publicationtype | article | de |
dc.subject.rswk | Biophysik | de |
dc.subject.rswk | Haarnadelschleife | de |
dc.subject.rswk | Hochdruckchemie | de |
dc.subject.rswk | Fluoreszenz-Resonanz-Energie-Transfer | de |
dc.subject.rswk | Synuclein <alpha-> | de |
dcterms.accessRights | open access | - |
eldorado.secondarypublication | true | de |
eldorado.secondarypublication.primaryidentifier | https://doi.org/10.1002/chem.202002119 | de |
eldorado.secondarypublication.primarycitation | Chemistry - a european journal. Vol. 26. 2020, issue 48, pp 10987-10991. Special issue: 8th EuChemS Chemistry Congress | de |
Appears in Collections: | Physikalische Chemie |
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chem.202002119.pdf | 1.25 MB | Adobe PDF | View/Open |
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