EXCLI Journal 2017;16:229-235 – ISSN 1611-2156 Received: June 06, 2016, accepted: February 09, 2017, published: March 08, 2017 S1 Supplementary data to: PARADOXICAL EFFECT OF MINOCYCLINE ON ESTABLISHED NEUROPATHIC PAIN IN RAT Malek Zarei1, Masoumeh Sabetkasaei1, Taraneh Moini-Zanjani1* 1 Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran * Taraneh Moini-Zanjani, Ph.D, Assistant Professor, Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Evin, Daneshjou Blvd, Koudakyar Ave, Tehran, Iran. Tel: +982122439969, Fax: +982122439969, P.O.BOX: 19395-4719, Postcode: 19857-17443 E-mail: tzanjani@yahoo.com, t.moini@sbmu.ac.ir http://dx.doi.org/10.17179/excli2016-434 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). Table 1: Data obtained from Paw withdrawal threshold in response to Von Frey filaments in different days and various groups when minocycline injected before nerve injury. Mechanical Allodynia Day 0 1 3 5 7 10 14 Sham 60 60 60 60 60 60 60 Control 60 37.33 ± 7.16 21.50 ± 8.14 10.17 ± 1.04 7.67 ± 80 5.00 ± .68 5.67 ± .95 M10 60 34.66 ± 8.40 32.83 ± 8.96 37.33 ± 7.16 40.33 ± 9.11 41.17 ± 8.58 43 ± 7.6 M20 60 33.67 ± 8.5 35.50 ± 7.94 43.00 ± 7.6 48.67 ± 7.16 52.50 ± 7.5 54.33 ± 5.66 M30 60 54.33 ± 5.66 48.67 ± 7.16 52.50 ± 7.5 54.33 ± 5.66 60 60 M40 60 54.33 ± 5.66 60 54.33 ± 5.66 54.33 ± 5.66 60 60 M10= Minocycline 10 mg/kg M20= Minocycline 20 mg/kg M30= Minocycline 30 mg/kg M40= Minocycline 40mg/kg. Data were presented as mean ± S.D. EXCLI Journal 2017;16:229-235 – ISSN 1611-2156 Received: June 06, 2016, accepted: February 09, 2017, published: March 08, 2017 S2 Table 2: Data obtained from Paw withdrawal latency in response to radiant heat in different days and various groups when minocycline injected before nerve injury. Thermal Hyperalgesia - pre Day 0 1 3 5 7 10 14 Sham 19.72 ± .09 18.98 ± .43 19.66 ± .15 19.49 ± .3 19.39 ± .29 19.42 ± .23 19.14 ± .49 Control 19.08 ± .54 10.45 ± 1.41 10.05 ± 2.14 10.83 ± 2.43 11.65 ± 2.08 11.96 ± 2.02 11.89 ± 2.26 M10 19.11 ± .10 12.14 ± .52 15.15 ± .50 17.17 ± .36 17.31 ± .55 17.88 ± .53 18.27 ± .37 M20 19.36 ± .18 14.98 ± .43 16.34 ± .54 17.08 ± .67 17.25 ± .64 18.12 ± .68 17.91 ± .53 M30 18.97 ± .29 17.92 ± .63 17.78 ± .33 19.19 ± .20 18.54 ± .53 17.60 ± 1.19 17.85 ± .37 M40 19.37 ± .25 17.88 ± .50 18.45 ± .54 17.92 ± .63 18.39 ± .51 18.17 ± .56 18.30 ± .35 M10= Minocycline 10 mg/kg M20= Minocycline 20 mg/kg M30= Minocycline 30 mg/kg M40= Minocycline 40mg/kg. Data were presented as mean ± S.D. Table 3: Data obtained from Paw withdrawal threshold in response to Von Frey filaments in different days and various groups when minocycline administered after nerve injury. Mechanical Allodynia Day 0 1 3 5 7 10 14 Sham 54.33 ± 5.67 60 54.33 ± 5.67 60 60 54.33 ± 5.67 60 Control 60 37.33 ± 7.17 19.67 ± 2.93 10.17 ± 1.05 8 ± .73 7.67 ± .80 6.33 ± .61 M10 60 35.50 ± 7.94 19.67 ± 2.93 12.50 ± 1.12 7.67± .80 10.17 ± 1.05 8.00 ± .73 M20 54.33 ± 5.67 31.66 ± 5.66 22.33 ± 2.32 14.33 ± 2.54 8.33 ± .61 11.00 ± 1.32 8.33 ± .80 M30 60 35.50 ± 7.94 23.33 ± 2.67 15.83 ± 3.35 9.17 ± 1.28 11.00 ± 1.32 9.50 ± 1.26 M40 60 35.50 ± 7.94 24.17 ± 1.83 16.17 ± 3.21 11.00 ± 1.32 10.17 ± 1.05 9.17 ± 1.38 M10= Minocycline 10 mg/kg M20= Minocycline 20 mg/kg M30= Minocycline 30 mg/kg M40= Minocycline 40mg/kg. Data were presented as mean ± S.D. EXCLI Journal 2017;16:229-235 – ISSN 1611-2156 Received: June 06, 2016, accepted: February 09, 2017, published: March 08, 2017 S3 Table 4: Data obtained from Paw withdrawal threshold in response to Von Frey filaments in different days and various groups when minocycline injected after nerve injury. Mechanical Allodynia - post Day 0 7 10 14 Sham 54.33 ± 5.67 60 54.33 ± 5.67 54.33 ± 5.67 Control 54.33 ± 5.67 10.33 ± 3.20 8.67 ± 3.49 8.00 ± 3.61 M10 60 16.67 ± 3.16 19.67 ± 2.93 29.83 ± 6.30 M20 54.33 ± 5.67 20.50 ± 2.46 20.67 ± 3.37 22.33 ± 5.67 M30 54.33 ± 5.67 14.50 ± 2.74 21.50 ± 8.15 25.33 ± 7.42 M40 60 21.50 ± 2.91 29.83 ± 6.29 32.83 ± 8.96072 M10= Minocycline 10 mg/kg M20= Minocycline 20 mg/kg M30= Minocycline 30 mg/kg M40= Minocycline 40mg/kg. Data were presented as mean ± S.D. Table 5: Data obtained from Paw withdrawal latency in response to radiant heat in different days and various groups when minocycline administered after nerve injury. Thermal Hyperalgesia - post Day 0 1 3 5 7 10 14 Sham 18.88 ± .32 19 ± .40 18.83 ± .37 18.94 ± .47 18.90 ± .35 18.57 ± .49 18.68 ± .32 Control 18.89 ± .29 10.73 ± .50 11.13 ± .55 11.18 ± 1.08 10.80 ± .4 11.37 ± 1.37 12.15 ± .88 M10 18.51 ± .36 11.18 ± .38 11.02 ± .76 12.60 ± .65 12.46 ± .79 12.59 ± 1.22 13.02 ± 1 M20 19.11 ± .44 11.23 ± .86 11.93 ± 1 11.21 ± .61 12.17 ± .54 12.77 ± .96 13.13 ± 1.13 M30 18.80 ± .22 11.71 ± .55 12.69 ± .44 13.18 ± .7 12.40 ± 1.03 13.46 ± 1.21 13.67 ± .83 M40 18.88 ± .29 12.21 ± .7 12.49 ± .93 13.24 ± .37 12.28 ± .85 13.62 ± .78 13.69 ± .87 M10= Minocycline 10 mg/kg M20= Minocycline 20 mg/kg M30= Minocycline 30 mg/kg M40= Minocycline 40mg/kg. Data were presented as mean ± S.D. EXCLI Journal 2017;16:229-235 – ISSN 1611-2156 Received: June 06, 2016, accepted: February 09, 2017, published: March 08, 2017 S4 Table 6: Data obtained from Paw withdrawal latency in response to radiant heat in different days and various groups when minocycline administered after nerve injury. Thermal Hyperalgesia - post Day 0 7 10 14 Sham 18.77 ± .17 19.66 ± .15 18.98 ± .44 19.49 ± .31 Control 18.73 ± .53 10.85 ± 1.76 11.27 ± 1.85 11.73 ± 1.71 M10 18.85 ± .31 11.35 ± .71 12.25 ± 1.22 12.14 ± 1.14 M20 18.97 ± .46 10.58± .53 11.80 ± .86 12.36 ± 1.25 M30 18.23 ± .21 12.92 ± 1.61 14.48 ± 1.41 14.17 ± 1.65 M40 19.17 ± .4 12.68 ± .91 14.02 ± 1.17 14.52 ± .57 M10= Minocycline 10 mg/kg M20= Minocycline 20 mg/kg M30= Minocycline 30 mg/kg M40= Minocycline 40mg/kg. Data were presented as mean ± S.D.