Nabu, SunantaLawung, RatanaIsarankura-Na-Ayudhya, PatchareeRoytrakul, SittirukDolprasit, SupamasSengyee, SineenartIsarankura-Na-Ayudhya, ChartchalermPrachayasittikul, Virapong2018-02-052018-02-052017-11-081611-2156http://hdl.handle.net/2003/3635610.17877/DE290R-18357Neisseria gonorrhoeae strains displaying reduced susceptibility and resistance to extended-spectrum cephalo- sporins (ESCs) are major public health concerns. Although resistance mechanisms of ESCs have extensively been studied, the proteome-wide investigation on the biological response to the antibiotic stress is still limited. Herein, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS analy- sis was applied to investigate the global protein expression under ESC stresses of ESC-susceptible and ESC- reduced susceptible N. gonorrhoeae strains. Upon exposure to ceftriaxone, 14 and 21 proteins of ESC- susceptible and ESC-reduced susceptible strains, respectively, were shown to be differentially expressed. In the meanwhile, differential expressions of 13 and 17 proteins were detected under cefixime stress for ESC- susceptible and ESC-reduced susceptible strains, respectively. ESC antibiotics have been proven to trigger the expression of several proteins implicated in a variety of biological functions including transport system, energy metabolism, stress response and pathogenic virulence factors. Interestingly, macrophage infectivity potentiators (Ng-MIP) showed increased expression for ESC-reduced susceptible strain under ESC stress. The altered ex- pression of Ng-MIP was found to be a unique response to ESC stresses. Our finding proposes a broad view on proteomic changes in N. gonorrhoeae in response to ESC antibiotics that provides further insights into the gono- coccal antimicrobial resistance and physiological adaptation mechanism.enEXCLI Journal;Vol. 16 2017http://creativecommons.org/licenses/by/4.0/Neisseria gonorrhoeaeExtended-spectrum cephalosporinsAntimicrobial resistanceProteomics610article (journal)