Ko, Eun-YiYoon, Weon-JongLee, Hae-WonHeo, Soo-JinKo, Young-HwanFernando, I.P. ShanuraCho, KichulLee, Chi-HeonHur, Sung-PyoCho, Su-HyeonAhn, GinnaeKim, DaekyungKim, Kil-Nam2016-11-242016-11-242016-06-281611-2156http://hdl.handle.net/2003/3561810.17877/DE290R-17659The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it’s anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced IκB-α degradation and p50 NF-κB activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPS-activated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-κB.enEXCLI Journal;Vol. 15, 2016https://creativecommons.org/licenses/by/4.0/supercritical fluid extractionlinoleic acidIshige okamuraeanti-inflammatoryNF- κB61010.17179/excli2016-337article (journal)