High yield functional expression, purification, and reconstitution of human sodium/D-glucose cotransporter 1 (hSGLT1) in Pichia pastoris & pilot studies for photoaffinity labeling of functional domains of SGLT1

Tyagi, Navneet Kumar2005-05-312005-05-312005-05-202005-05-17http://hdl.handle.net/2003/2135610.17877/DE290R-7860enUniversität DortmundCotransporter proteinSodium/D-glucose cotransporter1 (SGLT1)Heterologous expressionPichia pastorisEscherichia coliMessenger RNARibonuclease E (RNase E) ReconstitutionProteoliposomesAlpha-methyl-D-glucopyranosideD-glucosePhlorizinArbutinPhotoaffinity labeling3-AzidophlorizinBrush border membrane vesiclesMALDI mass spectrometry540High yield functional expression, purification, and reconstitution of human sodium/D-glucose cotransporter 1 (hSGLT1) in Pichia pastoris & pilot studies for photoaffinity labeling of functional domains of SGLT1doctoral thesis