Siedentop, RegineDziennus, MarlonLütz, StephanRosenthal, Katrin2025-03-072025-03-072023-02-10http://hdl.handle.net/2003/4352010.17877/DE290R-25353Designing a feasible in vitro enzyme cascade is a challenging task that is often preceded by optimization steps. Computational methods can increase the efficiency of the optimization process by providing a deeper insight into the system. In this study, bottlenecks of a farnesyl pyrophosphate-producing in vitro enzyme cascade were determined by a combination of in silico modeling and wet-lab experiments. ATP and the precursor-isomerizing enzyme isopentenyl diphosphate δ-isomerase were identified as limitations of the in vitro enzyme cascade.enChemie - Ingenieur - Technik; 95(4)https://creativecommons.org/licenses/by-nc/4.0/BiocatalysisEnzyme cascadesIn vitro biotransformationsKinetic modelsTerpenes660ResearchArticle