Ternes, Ana Paula LausmannZemolin, Ana Paulada Cruz, Litiele Cezarda Silva, Gustavo FelipeSaidelles, Ana Paula Fleigde Paula, Mariane TrindadeWagner, CarolineGolombieski, Ronaldo MedeirosFlores, Érico Marlon de MoraesPicoloto, Rochele SogariPereira, Antônio BatistaFranco, Jeferson LuisPosser, Thaís2015-04-202015-04-202014-11-211611-2156http://hdl.handle.net/2003/3400610.17877/DE290R-7402Embryonic animals are especially susceptible to metal exposure. Manganese (Mn) is an es-sential element, but in excess it can induce toxicity. In this study we used Drosophila mela-nogaster as an embryonic model to investigate biochemical and behavioral alterations due to Mn exposure. Flies were treated with standard medium supplemented with MnCl2 at 0.1 mM, 0.5 mM or 1 mM from the egg to the adult stage. At 0.5 mM and 1 mM Mn, newly ecloded flies showed significantly enhanced locomotor activity when assessed by negative geotaxis behavior. In addition, a significant increase in Mn levels (p < 0.0001) was observed, while Ca, Fe, Cu, Zn and S levels were significantly decreased. A significant drop in cell viability occurred in flies exposed to 1 mM Mn. There was also an induction of reactive oxygen species at 0.5 mM and 1 mM Mn (p < 0.05). At 1 mM, Mn increased Catalase (p < 0.005), Superoxide Dismutase (p < 0.005) and Hsp83 (p < 0.0001) mRNA expression, without altering Catalase or Superoxide Dismutase activity; the activity of Thioredoxin reductase and Glutatione-S-transferase enzymes was increased. Mn treatment did not alter ERK or JNK1/2 phosphorylation, but at 1 mM caused an inhibition of p38MAPK phosphorylation. Together these data suggest mechanisms of adaptation in the fly response to Mn exposure in embryonic life.enEXCLI Journal ; Vol. 13, 2014ManganeseDrosophila melanogasterMAPKoxidative stressThioredoxin reductase610article (journal)