Authors: | Günther, Patrick |
Title: | Structural investigation of cholesterol homeostasis and bacterial toxins |
Language (ISO): | en |
Abstract: | Membrane proteins regulate a variety of processes that are critical for living organisms. They participate in cell-cell communication, catalyze reactions in or at the membrane, are involved in transmitting signals from the environment into the cell, and can transport molecules across membranes. Approximately 60% of all clinically approved drugs target membrane proteins, underscoring their importance. In order to understand the function of membrane proteins and to design more targeted drugs, determining their precise three-dimensional structures is required. In this PhD project, I aimed to structurally characterize two membrane protein complexes involved in the regulation of cholesterol homeostasis – the Scap-Insig and HMGCR-UBIAD1 complexes – and the type VI secretion system (T6SS) effector RhsA. My PhD work showcases that biochemical studies combined with structural determination by cryo-EM provides valuable insights into molecular processes that occur in or at the membrane and is of utmost pharmacological interest. |
Subject Headings: | Cryo-EM Single particle analysis Membrane proteins Bacterial toxins Type VI secretion system Cholesterol |
Subject Headings (RSWK): | Elektronenmikroskopie Membranproteine |
URI: | http://hdl.handle.net/2003/42108 http://dx.doi.org/10.17877/DE290R-23941 |
Issue Date: | 2023 |
Appears in Collections: | Chemische Biologie |
Files in This Item:
File | Description | Size | Format | |
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Dissertation_Guenther.pdf | DNB | 26.14 MB | Adobe PDF | View/Open |
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