Eldorado - Repositorium der TU Dortmund

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Aktuellste Veröffentlichungen

Acute exercise rewires the proteomic landscape of human immune cells
(Springer Science and Business Media LLC, 2026-01-02) Walzik, David; Joisten, Niklas; Metcalfe, Alan J.; Proschinger, Sebastian; Schenk, Alexander; Wenzel, Charlotte; Henneberg, Alessa L.; Schneider, Martin; Calderazzo, Silvia; Groll, Andreas; Watzl, Carsten; Opitz, Christiane A.; Helm, Dominic; Zimmer, Philipp
The positive effect of exercise on the immune system is widely acknowledged, but the molecular response of immune cells to exercise remains largely unknown. Here, we perform mass-spectrometry-based proteomic analysis on peripheral blood mononuclear cells (PBMC) at a depth of >6000 proteins. Comparing high-intensity interval exercise (HIIE) and moderate-intensity continuous exercise (MICE), matched for time and workload, we identify versatile changes in the proteomic makeup of PBMCs and reveal profound alterations, related to effector function and immune cell activation pathways within one hour following exercise. These changes are more pronounced after HIIE compared to MICE and occur despite identical immune cell mobilization patterns between the two exercise conditions. We further identify an immunoproteomic signature that effectively predicts cardiorespiratory fitness, thus allowing insights into potential exercise-triggered adaptations and immunological health benefits that are mediated by exercise. This study provides a reliable data resource that expands our knowledge on how exercise modulates the immune system, and delivers biological evidence supporting the WHO 2020 guidelines, which highlight exercise intensity as a relevant factor to maintain health.
Engineering Pseudomonas taiwanensis VLB120 for regio- and stereospecific hydroxylation of l-lysine fueled by the Weimberg pathway
(Springer Science and Business Media LLC, 2026-02-19) Nerke, Philipp; Handke, Julian; Hubmann, Georg; Lütz, Stephan
Background Hydroxy-l-lysines are versatile chiral building blocks and can be obtained by hydroxylation of the amino acid l-lysine. The conversion is catalyzed by α-ketoglutarate-dependent lysine dioxygenases (KDOs), which belong to the superfamily of Fe2+/α-ketoglutarate-dependent oxygenases. These enzymes are highly regio- and stereoselective; however, they require α-ketoglutarate (α-KG) as a cosubstrate. Apart from the costly direct addition of α-KG, it can be generated via cellular metabolism from inexpensive and renewable carbon sources, such as d-xylose. Therefore, we engineered a Pseudomonas taiwanensis VLB120 chassis to efficiently convert l-lysine to hydroxy-l-lysine using KDOs with the supply of α-KG from d-xylose as the sole carbon source via the Weimberg pathway. Results For the generation of a suitable whole-cell biocatalyst, we investigated the l-lysine catabolism of P. taiwanensis VLB120 and created a mutant strain that is deficient in l-lysine catabolism to minimize l-lysine degradation and to facilitate complete conversion via the biotransformation reaction. Next, a library of KDO genes was heterologously expressed in the engineered chassis strain P. taiwanensis VLB120∆C∆3. The hydroxylation of l-lysine was assessed in biotransformations with growing cells and d-xylose to supply α-KG via the Weimberg pathway. Hydroxy-l-lysine was successfully produced by strains harboring KDOs that hydroxylate the C-4 position of l-lysine. We further explored the three most promising whole-cell biocatalysts and investigated the influence of increased concentrations of the substrate l-lysine and the metal cofactor Fe2+. Finally, the engineered strain expressing a KDO from Flavobacterium species was grown in stirred-tank bioreactors and was able to produce 8.7 ± 0.3 g L−1 hydroxy-l-lysine with a space-time yield of 98.6 ± 3.4 mg L h−1 and a specific product yield on biocatalyst (YHyl/X) of 1.68 ± 0.07 g gCDW−1. The supply of α-KG via the Weimberg pathway proved very efficient, as approximately every second molecule of d-xylose which was converted and entered the central carbon metabolism was used for the biotransformation reaction (YHyl/Xyl,net = 0.48 ± 0.02 mol mol−1). Conclusions We successfully established a whole-cell biocatalyst for the synthesis of hydroxy-l-lysine from l-lysine and d-xylose and demonstrated multigram-scale production with our engineered strain. Our work lays the foundation for whole-cell bioprocesses utilizing Fe2+/α-ketoglutarate-dependent oxygenases fueled by the Weimberg pathway.
Impact of flood risks on service disruptions of critical infrastructures by the example of the Euskirchen County, Germany
(2026-03-02) Fuchs, Marisa; Oriwol, Jennifer; Zimny, Matthias; Lenze, Lara; Greiving, Stefan
The increasing severity of flood events poses growing risks to critical infrastructures (CI), with failures often triggering cascading effects across sectors. Despite this, flood risk management (FRM) frequently overlooks service disruptions of CI and systemic interdependencies between infrastructure sectors because of its place-based approach. This study addresses this gap by developing and applying an integrated methodological framework that combines GIS-based flood risk analysis and qualitative criticality assessment. Focusing on Euskirchen County, Germany, the study analyses the exposure, vulnerability, and criticality of CI—specifically in the energy, (tele)communications, emergency service, and transport sectors—under extreme flood scenarios. Expert interviews and workshops informed the identification of cascading failure chains and potential intervention points. Findings reveal significant systemic risks, particularly stemming from power outages, and highlight key interdependencies between infrastructure sectors. The results provide actionable insights for spatial planners and emergency managers, including recommendations for strategic CI siting and contingency planning. By integrating scientific rigor with practical relevance, the study contributes to the advancement of risk-based spatial planning and supports the development of resilient infrastructure systems in the face of increasing flood risks.
Finite element approximation of data-driven problems in conductivity
(Springer Science and Business Media LLC, 2024-10-10) Schiemann, Annika; Meyer, Christian
This paper is concerned with the finite element discretization of the data driven approach according to Kirchdoerfer and Ortiz (Comput Methods Appl Mech Eng 304:81–101, 2016) for the solution of PDEs with a material law arising from measurement data. To simplify the setting, we focus on a scalar diffusion problem instead of a problem in elasticity. It is proven that the data convergence analysis from Conti et al. (Arch Ration Mech Anal 229(1):79–123, 2018) carries over to the finite element discretization as long as -conforming finite elements such as the Raviart–Thomas element are used. As a corollary, minimizers of the discretized problems converge in data in the sense of Conti et al., as the mesh size tends to zero and the approximation of the local material data set gets more and more accurate. We moreover present several heuristics for the solution of the discretized data driven problems, which is equivalent to a quadratic semi-assignment problem and therefore NP-hard. We test these heuristics by means of three examples and it turns out that the “classical” alternating projection method according to Kirchdoerfer and Ortiz is superior w.r.t. the ratio of accuracy and computational time.
Fachlichkeit und Professionalisierung des Personals in der Kinder- und Jugendhilfe
(Springer Science and Business Media LLC, 2024-06-06) Birkel-Barmsen, Janine; Meiner-Teubner, Christiane
ZusammenfassungErgebnisse der Kinder- und Jugendhilfestatistik zeigen, dass das Qualifikationsgefüge in der Kinder- und Jugendhilfe trotz enormem Personalzuwachs in den vergangenen 15 Jahren stabil geblieben ist. Auf der Grundlage jüngster Vorausberechnungen ist bereits heute absehbar, dass in Westdeutschland auch im kommenden Jahrzehnt ein hoher Fachkräftemangel bestehen wird. Bereits seit etwa zehn Jahren kommt es immer wieder zu Reformen in den Ausbildungen, um mehr Interessierte für die Ausbildung zu gewinnen und zuletzt reagierte die Mehrzahl der Länder mit Öffnungen der Fachkräftekataloge im Bereich der Frühen Bildung, um zusätzliches Personal zu gewinnen. Diese Maßnahmen tragen zwar einerseits zur Reduzierung des Fachkräftemangels bei, bergen aber auch verschiedene Gefahren und Risiken.