Mass transfer of proteins in aqueous two-phase systems

dc.contributor.authorKaplanow, Irene
dc.contributor.authorGörzgen, Fabian
dc.contributor.authorMerz, Juliane
dc.contributor.authorSchembecker, Gerhard
dc.date.accessioned2019-05-23T10:31:13Z
dc.date.available2019-05-23T10:31:13Z
dc.date.issued2019-03-06
dc.description.abstractAqueous Two-Phase Extraction is known to be a gentle separation technique for biochemical molecules where product partitioning is fast. However, the reason for the high mass transfer rates has not been investigated, yet. Many researchers claim that the low interfacial tension facilitates the formation of very small droplets and with it a large interfacial area causing a fast partitioning. However, an experimental evidence for this hypothesis has not been published yet. In this study, the mass transfer coefficients of two proteins, namely lysozyme and bromelain, were determined by providing a defined interfacial area for partitioning. Compared to low molecular weight solutes the mass transfer coefficient for the proteins investigated was small proving for the first time that the large interfacial area and not fast diffusion seems to be the reason for fast protein partitioning.en
dc.identifier.urihttp://hdl.handle.net/2003/38069
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-20051
dc.language.isoende
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc660
dc.subject.rswkExtraktionde
dc.titleMass transfer of proteins in aqueous two-phase systemsen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationKaplanow, I, Goerzgen, F, Merz, J, Schembecker, G (2019) Mass transfer of proteins in aqueous two-phase systems. Scientific reports 9, Article number 3692de
eldorado.secondarypublication.primaryidentifierdoi:10.1038/s41598-019-39797-9de

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