Ribozyme-mediated RNA synthesis and replication in a model Hadean microenvironment

Simple item page
dc.contributor.authorSalditt, Annalena
dc.contributor.authorKarr, Leonie
dc.contributor.authorSalibi, Elia
dc.contributor.authorLe Vay, Kristian
dc.contributor.authorBraun, Dieter
dc.contributor.authorMutschler, Hannes
dc.date.accessioned2024-08-15T10:39:33Z
dc.date.available2024-08-15T10:39:33Z
dc.date.issued2023-03-17
dc.description.abstractEnzyme-catalyzed replication of nucleic acid sequences is a prerequisite for the survival and evolution of biological entities. Before the advent of protein synthesis, genetic information was most likely stored in and replicated by RNA. However, experimental systems for sustained RNA-dependent RNA-replication are difficult to realise, in part due to the high thermodynamic stability of duplex products and the low chemical stability of catalytic RNAs. Using a derivative of a group I intron as a model for an RNA replicase, we show that heated air-water interfaces that are exposed to a plausible CO2-rich atmosphere enable sense and antisense RNA replication as well as template-dependent synthesis and catalysis of a functional ribozyme in a one-pot reaction. Both reactions are driven by autonomous oscillations in salt concentrations and pH, resulting from precipitation of acidified dew droplets, which transiently destabilise RNA duplexes. Our results suggest that an abundant Hadean microenvironment may have promoted both replication and synthesis of functional RNAs.en
dc.identifier.urihttp://hdl.handle.net/2003/42649
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-24486
dc.language.isoende
dc.relation.ispartofseriesNature communications;14
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/de
dc.subjectChemical origin of lifeen
dc.subjectRibozymesen
dc.subjectSynthetic biologyen
dc.subject.ddc570
dc.subject.ddc540
dc.titleRibozyme-mediated RNA synthesis and replication in a model Hadean microenvironmenten
dc.typeTextde
dc.type.publicationtypeArticlede
dcterms.accessRightsopen access
eldorado.openaire.projectidentifierinfo:eu-repo/grantAgreement/EC/H2020/802000/EU/Resurrecting LUCA - Engineering of RNA-encoded Cellular Life Using Dual Evolution and Intergenomic Transplantation/RiboLife
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationSalditt, A., Karr, L., Salibi, E. et al. Ribozyme-mediated RNA synthesis and replication in a model Hadean microenvironment. Nat Commun 14, 1495 (2023). https://doi.org/10.1038/s41467-023-37206-4de
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1038/s41467-023-37206-4de

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41467-023-37206-4.pdf
Size:
1.32 MB
Format:
Adobe Portable Document Format
Description:
DNB
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Chemische Biologie