Agar plate-based screening approach for the identification of enzyme-catalyzed oxidations

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dc.contributor.authorKinner, Alina
dc.contributor.authorLütz, Stephan
dc.contributor.authorRosenthal, Katrin
dc.date.accessioned2024-01-08T14:03:27Z
dc.date.available2024-01-08T14:03:27Z
dc.date.issued2022-09-29
dc.description.abstractBiocatalytic oxidation reactions are in high demand. Among the applied enzymes, the heme-thiolate enzyme subfamily of unspecific peroxygenases (UPOs), which relies on hydrogen peroxide as cosubstrate and oxidant, has generated great interest. Almost two decades after their first description, databases provide thousands of putative UPO sequences, but only a few enzymes have been characterized. To address this gap, efficient screening methods for the identification of novel UPOs are necessary. Here, a new screening strategy is presented based on solid cultivation of wild-type fungi. The identification of a promising candidate strain highlights the applicability of this approach.en
dc.identifier.urihttp://hdl.handle.net/2003/42268
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-24105
dc.language.isoende
dc.relation.ispartofseriesChemie - Ingenieur - Technik;94(11)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/de
dc.subjectABTSen
dc.subjectActivity screeningen
dc.subjectLaccaseen
dc.subjectUnspecific peroxygenaseen
dc.subjectWild-type fungien
dc.subject.ddc660
dc.titleAgar plate-based screening approach for the identification of enzyme-catalyzed oxidationsen
dc.typeTextde
dc.type.publicationtypeArticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationKinner, A., Lütz, S. and Rosenthal, K. (2022), Agar Plate-Based Screening Approach for the Identification of Enzyme-Catalyzed Oxidations. Chemie Ingenieur Technik, 94: 1853-1859. https://doi.org/10.1002/cite.202200084de
eldorado.secondarypublication.primaryidentifierDOI https://doi.org/10.1002/cite.202200084de

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