Probing the electronic structure of transition metal ion centres in proteins by coherent Raman detected electron paramagnetic resonance spectroscopy

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dc.contributor.authorSuter, Dieter
dc.contributor.authorBingham, Stephen J.
dc.contributor.authorBörger, Birgit
dc.contributor.authorThomson, Andrew J.
dc.contributor.authorGutschank, Jörg
dc.date.accessioned2008-05-30T11:36:23Z
dc.date.available2008-05-30T11:36:23Z
dc.date.issued2000-02
dc.description.abstractThe simultaneous excitation of a paramagnetic sample with optical (laser) and microwave radiation can cause an amplitude or phase modulation of the transmitted light at the microwave frequency. The detection of this modulation indicates the presence of coupled optical and electron paramagnetic resonance (EPR) transitions in the sample. Here we report the first application of this technique to a biomolecule: the blue copper centre of Pseudomonas aeruginosa azurin. Using optical excitation at 686 nm, in the thiol to copper(II) charge transfer band, we measure a coherent Raman-detected EPR spectrum of a frozen aqueous solution. Its lineshape is characteristic of the magnetic circular dichroism along each principal g-value axis. This information allows electronic and structural models of transition metal ion centres in proteins to be tested.en
dc.identifier.citationBingham, Stephen J.; Börger, Birgit; Gutschank, Jörg; Suter, Dieter, Thomson, Andrew J.: Probing the electronic structure of transition metal ion centres in proteins by coherent Raman detected electron paramagnetic resonance spectroscopy. In: Journal of Biological Inorganic Chemistry Nr. 1, Jg. 5(2000), S. 30-35.de
dc.identifier.doi10.1002/(SICI)1522-2594(200002)43:2<259::AID-MRM13>3.0.CO;2-P
dc.identifier.urihttp://hdl.handle.net/2003/25352
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-2687
dc.identifier.urlhttp://dx.doi.org/10.1007/s007750050004
dc.language.isoende
dc.publisherSpringerde
dc.rights© 2000 Springerde
dc.subject.ddc530
dc.titleProbing the electronic structure of transition metal ion centres in proteins by coherent Raman detected electron paramagnetic resonance spectroscopyen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsrestricted
eldorado.identifier.urlhttp://e3.physik.tu-dortmund.de/~suter/eprints/Raman-EPR_of_metal_proteins.pdf

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