Acute exercise boosts NAD+ metabolism of human peripheral blood mononuclear cells

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dc.contributor.authorWalzik, David
dc.contributor.authorJoisten, Niklas
dc.contributor.authorSchenk, Alexander
dc.contributor.authorTrebing, Sina
dc.contributor.authorSchaaf, Kirill
dc.contributor.authorMetcalfe, Alan J
dc.contributor.authorSpiliopoulou, Polyxeni
dc.contributor.authorHiefner, Johanna
dc.contributor.authorMcCann, Adrian
dc.contributor.authorWatzl, Carsten
dc.contributor.authorUeland, Per Magne
dc.contributor.authorGehlert, Sebastian
dc.contributor.authorWorthmann, Anna
dc.contributor.authorBrenner, Charles
dc.contributor.authorZimmer, Philipp
dc.date.accessioned2025-11-05T12:48:13Z
dc.date.available2025-11-05T12:48:13Z
dc.date.issued2024-11-03
dc.description.abstractNicotinamide adenine dinucleotide (NAD+) coenzymes are the central electron carriers in biological energy metabolism. Low NAD+ levels are proposed as a hallmark of ageing and several diseases, which has given rise to therapeutic strategies that aim to tackle these conditions by boosting NAD+ levels. As a lifestyle factor with preventive and therapeutic effects, exercise increases NAD+ levels across various tissues, but so far human trials are mostly focused on skeletal muscle. Given that immune cells are mobilized and redistributed in response to acute exercise, we conducted two complementary trials to test the hypothesis that a single exercise session alters NAD+ metabolism of peripheral blood mononuclear cells (PBMCs). In a randomized crossover trial (DRKS00017686) with 24 young adults (12 female) we show that acute exercise increases gene expression and protein abundance of several key NAD+ metabolism enzymes with high conformity between high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT). In a longitudinal exercise trial (DRKS00029105) with 12 young adults (6 female) we confirm these results and reveal that – similar to skeletal muscle – NAD+ salvage is pivotal for PBMCs in response to exercise. Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme of NAD+ salvage pathway, displayed a pronounced increase in gene expression during exercise, which was accompanied by elevated intracellular NAD+ levels and reduced serum levels of the NAD+ precursor nicotinamide. These results demonstrate that acute exercise triggers NAD+ biosynthesis of human PBMCs with potential implications for immunometabolism, immune effector function, and immunological exercise adaptions.en
dc.identifier.urihttp://hdl.handle.net/2003/44085
dc.language.isoen
dc.relation.ispartofseriesBrain, behavior and immunity; 123
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectExerciseen
dc.subjectImmune cellen
dc.subjectMetabolismen
dc.subjectNicotinamide adenine dinucleotideen
dc.subjectNAD+en
dc.subjectPBMCen
dc.subject.ddc796
dc.subject.rswkTraining
dc.subject.rswkBlutzelle
dc.subject.rswkStoffwechsel
dc.titleAcute exercise boosts NAD+ metabolism of human peripheral blood mononuclear cellsen
dc.typeText
dc.type.publicationtypeArticle
dcterms.accessRightsopen access
eldorado.dnb.deposittrue
eldorado.doi.registerfalse
eldorado.secondarypublicationtrue
eldorado.secondarypublication.primarycitationDavid Walzik, Niklas Joisten, Alexander Schenk, Sina Trebing, Kirill Schaaf, Alan J Metcalfe, Polyxeni Spiliopoulou, Johanna Hiefner, Adrian McCann, Carsten Watzl, Per Magne Ueland, Sebastian Gehlert, Anna Worthmann, Charles Brenner, Philipp Zimmer, Acute exercise boosts NAD+ metabolism of human peripheral blood mononuclear cells, Brain, Behavior, and Immunity, Volume 123, 2025, Pages 1011-1023, https://doi.org/10.1016/j.bbi.2024.11.004.
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1016/j.bbi.2024.11.004

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