Rationalizing the optimization of detergents for membrane protein purification

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dc.contributor.authorUrner, Leonhard H.
dc.contributor.authorJunge, Florian
dc.contributor.authorFiorentino, Francesco
dc.contributor.authorEl-Baba, Tarick J.
dc.contributor.authorShutin, Denis
dc.contributor.authorNölte, Gideon
dc.contributor.authorHaag, Rainer
dc.contributor.authorRobinson, Carol V.
dc.date.accessioned2025-02-11T14:41:35Z
dc.date.available2025-02-11T14:41:35Z
dc.date.issued2023-03-10
dc.description.abstractMembrane protein purification by means of detergents is key to isolating membrane-bound therapeutic targets. The role of the detergent structure in this process, however, is not well understood. Detergents are optimized empirically, leading to failed preparations, and thereby raising costs. Here we evaluate the utility of the hydrophilic-lipophilic balance (HLB) concept, which was introduced by Griffin in 1949, for guiding the optimization of the hydrophobic tail in first-generation, dendritic oligoglycerol detergents ([G1] OGDs). Our findings deliver qualitative HLB guidelines for rationalizing the optimization of detergents. Moreover, [G1] OGDs exhibit strongly delipidating properties, regardless of the structure of the hydrophobic tail, which delivers a methodological enabling step for investigating binding strengths of endogenous lipids and their role for membrane protein oligomerization. Our findings will facilitate the analysis of challenging drug targets in the future.en
dc.identifier.urihttp://hdl.handle.net/2003/43460
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-25291
dc.language.isoen
dc.relation.ispartofseriesChemistry - a European journal; 29(30)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectdetergenten
dc.subjectHLBen
dc.subjectlipiden
dc.subjectmembraneen
dc.subjectproteinen
dc.subject.ddc570
dc.subject.ddc540
dc.titleRationalizing the optimization of detergents for membrane protein purificationen
dc.typeText
dc.type.publicationtypeResearchArticle
dcterms.accessRightsopen access
eldorado.secondarypublicationtrue
eldorado.secondarypublication.primarycitationL. H. Urner, F. Junge, F. Fiorentino, T. J. El-Baba, D. Shutin, G. Nölte, R. Haag, C. V. Robinson, Chem. Eur. J. 2023, 29, e202300159.
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/chem.202300159

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