Design principles and applications of synthetic self-replicating RNAs

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dc.contributor.authorWagner, Alexander
dc.contributor.authorMutschler, Hannes
dc.date.accessioned2024-12-05T14:09:39Z
dc.date.available2024-12-05T14:09:39Z
dc.date.issued2023-06-01
dc.description.abstractWith the advent of ever more sophisticated methods for the in vitro synthesis and the in vivo delivery of RNAs, synthetic mRNAs have gained substantial interest both for medical applications, as well as for biotechnology. However, in most biological systems exogeneous mRNAs possess only a limited half-life, especially in fast dividing cells. In contrast, viral RNAs can extend their lifetime by actively replicating inside their host. As such they may serve as scaffolds for the design of synthetic self-replicating RNAs (srRNA), which can be used to increase both the half-life and intracellular concentration of coding RNAs. Synthetic srRNAs may be used to enhance recombinant protein expression or induce the reprogramming of differentiated cells into pluripotent stem cells but also to create cell-free systems for research based on experimental evolution. In this article, we discuss the applications and design principles of srRNAs used for cellular reprogramming, mRNA-based vaccines and tools for synthetic biology. This article is categorized under: RNA in Disease and Development > RNA in Disease RNA in Disease and Development > RNA in Development RNA Evolution and Genomics > RNA and Ribonucleoprotein Evolutionen
dc.identifier.urihttp://hdl.handle.net/2003/43089
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-24921
dc.language.isoen
dc.relation.ispartofseriesWiley interdisciplinary reviews : WIREs. RNA; 14(6)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectinduced pluripotent stem cellsen
dc.subjectRNA replicationen
dc.subjectRNA therapeuticsen
dc.subjectRNA vaccinesen
dc.subjectviral RNAsen
dc.subject.ddc570
dc.subject.ddc540
dc.titleDesign principles and applications of synthetic self-replicating RNAsen
dc.typeText
dc.type.publicationtypeArticle
dcterms.accessRightsopen access
eldorado.openaire.projectidentifierinfo:eu-repo/grantAgreement/EC/H2020/802000/EU/Resurrecting LUCA - Engineering of RNA-encoded Cellular Life Using Dual Evolution and Intergenomic Transplantation/RiboLife
eldorado.secondarypublicationtrue
eldorado.secondarypublication.primarycitationWagner, A., & Mutschler, H. (2023). Design principles and applications of synthetic self-replicating RNAs. WIREs RNA, 14(6), e1803. https://doi.org/10.1002/wrna.1803
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/wrna.1803

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