Synthesis and characterization of cationic hydrogels from thiolated copolymers for independent manipulation of mechanical and chemical properties of cell substrates

Simple item page
dc.contributor.authorPätzold, Florian
dc.contributor.authorStamm, Nils
dc.contributor.authorKamps, Dominic
dc.contributor.authorSpecht, Maria
dc.contributor.authorBolduan, Patrick
dc.contributor.authorDehmelt, Leif
dc.contributor.authorWeberskirch, Ralf
dc.date.accessioned2024-03-04T14:43:04Z
dc.date.available2024-03-04T14:43:04Z
dc.date.issued2022-02-20
dc.description.abstractCells sense both mechanical and chemical properties in their environment and respond to these inputs with altered phenotypes. Precise and selective experimental manipulations of these environmental cues require biocompatible synthetic materials, for which multiple properties can be fine-tuned independently from each other. For example, cells typically show critical thresholds for cell adhesion as a function of substrate parameters such as stiffness and the degree of functionalization. However, the choice of tailor-made, defined materials to produce such cell adhesion substrates is still very limited. Here, a platform of synthetic hydrogels based on well-defined thiolated copolymers is presented. Therefore, four disulfide crosslinked hydrogels of different composition by free radical polymerization are prepared. After cleavage with dithiothreitol, four soluble copolymers P1–P4 with 0–96% cationic monomer content are obtained. P1 and P4 are then combined with PEGDA3500 as a crosslinker, to fabricate 12 hydrogels with variable elasticity, ranging from 8.1 to 26.3 kPa and cationic group concentrations of up to 350 µmol cm−3. Systematic analysis using COS7 cells shows that all of these hydrogels are nontoxic. However, successful cell adhesion requires both a minimal elasticity and a minimal cationic group concentration.en
dc.identifier.urihttp://hdl.handle.net/2003/42374
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-24211
dc.language.isoende
dc.relation.ispartofseriesMacromolecular bioscience;22(5)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/de
dc.subjectcell adhesionen
dc.subjectCOS7 cellen
dc.subjecthydrogelen
dc.subjectMichael reactionen
dc.subjectprecursor polymeren
dc.subject.ddc540
dc.subject.rswkZelladhäsionde
dc.subject.rswkHydrogelde
dc.subject.rswkMichael-Additionde
dc.subject.rswkPräkursorde
dc.subject.rswkPolymerede
dc.titleSynthesis and characterization of cationic hydrogels from thiolated copolymers for independent manipulation of mechanical and chemical properties of cell substratesen
dc.typeTextde
dc.type.publicationtypeResearchArticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationPätzold, F., Stamm, N., Kamps, D., Specht, M., Bolduan, P., Dehmelt, L. and Weberskirch, R. (2022), Synthesis and Characterization of Cationic Hydrogels from Thiolated Copolymers for Independent Manipulation of Mechanical and Chemical Properties of Cell Substrates. Macromol. Biosci., 22: 2100453. https://doi.org/10.1002/mabi.202100453de
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/mabi.202100453de

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Macromolecular Bioscience - 2022 - Pätzold - Synthesis and Characterization of Cationic Hydrogels from Thiolated Copolymers.pdf
Size:
898.03 KB
Format:
Adobe Portable Document Format
Description:
DNB
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Organische Chemie