Expanding the genetic code for site-directed spin-labeling

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dc.contributor.authorBraun, Theresa
dc.contributor.authorDrescher, Malte
dc.contributor.authorSummerer, Daniel
dc.date.accessioned2019-06-19T07:01:15Z
dc.date.available2019-06-19T07:01:15Z
dc.date.issued2019
dc.description.abstractSite-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy enables studies of the structure, dynamics, and interactions of proteins in the noncrystalline state. The scope and analytical value of SDSL–EPR experiments crucially depends on the employed labeling strategy, with key aspects being labeling chemoselectivity and biocompatibility, as well as stability and spectroscopic properties of the resulting label. The use of genetically encoded noncanonical amino acids (ncAA) is an emerging strategy for SDSL that holds great promise for providing excellent chemoselectivity and potential for experiments in complex biological environments such as living cells. We here give a focused overview of recent advancements in this field and discuss their potentials and challenges for advancing SDSL–EPR studies.en
dc.identifier.urihttp://hdl.handle.net/2003/38105
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-20086
dc.language.isoende
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectNoncanonical amino acidsen
dc.subjectBioorthogonal chemistryen
dc.subjectSpin labelingen
dc.subjectProtein conformationen
dc.subjectEPR spectroscopyen
dc.subjectMacromolecular dynamicsen
dc.subject.ddc570
dc.subject.ddc540
dc.titleExpanding the genetic code for site-directed spin-labelingen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.openaire.projectidentifierinfo:eu-repo/grantAgreement/EC/H2020/723863/EU/Programmable Readers, Writers, and Erasers of the Epigenetic Cytosine Code/EPICODEde
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationBraun, T.; Drescher, M.; Summerer, D. Expanding the Genetic Code for Site-Directed Spin-Labeling. Int. J. Mol. Sci. 2019, 20, 373de
eldorado.secondarypublication.primaryidentifierdoi: 10.3390/ijms20020373.de

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