Theory and applications of wide field surface plasmon resonance microscopy for discrete particles detection
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Date
2025
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Abstract
Detecting and characterizing nano-objects with low concentrations, such as biological particles, is
a substantial challenge in analytical science. The wide-field surface plasmon resonance microscopy
(WF-SPRM) can detect individual nano-objects in solutions and gas media bound to the sensor
surface. Therefore, WF-SPRM can detect low nano-object concentrations because the image
contains several square millimeters. In this work, the fundamental parameters for building highly
sensitive WF-SPRM were optimized. WF-SPRM can detect individual nano-objects in solutions
and gas media. Therefore, we derived a discrete particle model of SPR to describe the SPR sensor
of discrete particle detection. Theoretical, numerical, and experimental analyses of the SPR
detection principle were performed by considering discrete particle detection. Additionally, the
influence on the SPR sensitivity of coating the gold/silver layer with a dielectric layer with varying
refractive index is also studied. Different polyelectrolyte brushes, like polyacrylic acid, polyacrylic
acid-polyethylene oxide, and polyacrylic acid/iodine, are used to validate the enhancement of the
SPR sensitivity. Validation experiments are performed using polystyrene and silica nanoparticles
of varying sizes. Finally, the surface plasmon coupling behavior between the localized surface
plasmons (LSPs) of different shapes and sizes of metal nanostructures and the propagating surface
plasmons (PSPs) of the metal surface is investigated by employing experimental, simulation, and
theoretical approaches.
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Keywords
Technology and thin film, Optics, Surface plasmon resonance
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Oberflächenplasmonresonanz