Application of cell-free protein synthesis for faster biocatalyst development

Simple item page
dc.contributor.authorRolf, Jascha
dc.contributor.authorRosenthal, Katrin
dc.contributor.authorLütz, Stephan
dc.date.accessioned2019-03-27T12:57:23Z
dc.date.available2019-03-27T12:57:23Z
dc.date.issued2019-02-19
dc.description.abstractCell-free protein synthesis (CFPS) has become an established tool for rapid protein synthesis in order to accelerate the discovery of new enzymes and the development of proteins with improved characteristics. Over the past years, progress in CFPS system preparation has been made towards simplification, and many applications have been developed with regard to tailor-made solutions for specific purposes. In this review, various preparation methods of CFPS systems are compared and the significance of individual supplements is assessed. The recent applications of CFPS are summarized and the potential for biocatalyst development discussed. One of the central features is the high-throughput synthesis of protein variants, which enables sophisticated approaches for rapid prototyping of enzymes. These applications demonstrate the contribution of CFPS to enhance enzyme functionalities and the complementation to in vivo protein synthesis. However, there are different issues to be addressed, such as the low predictability of CFPS performance and transferability to in vivo protein synthesis. Nevertheless, the usage of CFPS for high-throughput enzyme screening has been proven to be an efficient method to discover novel biocatalysts and improved enzyme variants.en
dc.identifier.urihttp://hdl.handle.net/2003/37963
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-19948
dc.language.isoende
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCell-free protein synthesis (CFPS)en
dc.subjectIn vitro protein synthesisen
dc.subjectE. coli extracten
dc.subjectBiocatalysisen
dc.subjectEnzymeen
dc.subjectHigh-throughput screeningen
dc.subjectSynthetic biologyen
dc.subjectProtein engineeringen
dc.subject.ddc660
dc.subject.rswkBiokatalysede
dc.subject.rswkSynthetische Biologiede
dc.titleApplication of cell-free protein synthesis for faster biocatalyst developmenten
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationRolf, J.; Rosenthal, K.; Lütz, S. Application of Cell-Free Protein Synthesis for Faster Biocatalyst Development. Catalysts 2019, 9, 190.de
eldorado.secondarypublication.primaryidentifierDOI: 10.3390/catal9020190de

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
catalysts-09-00190.pdf
Size:
2.55 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Arbeitsgruppe Bioverfahrenstechnik