Full metadata record
DC FieldValueLanguage
dc.contributor.authorKim, Haeng Hoon-
dc.contributor.authorKim, Hye Ran-
dc.contributor.authorKim, Jae Kwang-
dc.contributor.authorKim, Yeon Bok-
dc.contributor.authorKwon, Do Yeon-
dc.contributor.authorLee, Jeongyeo-
dc.contributor.authorPark, Sang Un-
dc.contributor.authorPark, Woo Tae-
dc.contributor.authorTuan, Pham Anh-
dc.date.accessioned2012-11-16T10:01:45Z-
dc.date.available2012-11-16T10:01:45Z-
dc.date.issued2012-11-16-
dc.identifier.issn1611-2156-
dc.identifier.urihttp://hdl.handle.net/2003/29771-
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-4958-
dc.description.abstractThe relationship between carotenoid accumulation and expression of carotenoid biosynthesis genes was investigated in the flowers, stems, young leaves, old leaves, and roots of Chinese cabbage (Brassica rapa subsp. pekinensis). Quantitative real-time PCR analysis showed that the mRNA levels of BrPSY, BrPDS, BrZDS, BrLCYB, BrLCYE, BrCHXB, and BrZEP leading to the production of carotenoids were highest in the flowers or the leaves and lowest in the roots of Chinese cabbage. In contrast, the mRNA expression of BrNCED, a gene involved in abscisic acid (ABA) biosynthesis, was highest in the roots. High-performance liquid chromatography revealed that carotenoids, namely, lutein and β-carotene, were distributed predominantly in the flowers and leaves, with very little in the underground organ, the roots. Specifically, old leaves contained 120.3 μg/g lutein and 103.93 μg/g β-carotene, which is the most potent dietary precursor of vitamin A. Moreover, we found a relatively large amount of cis isomers of β-carotene, namely, 9-cis β-carotene and 13-cis β-carotene, in Chinese cabbage. These results provide insight into carotenoid biosynthetic mechanisms in Chinese cabbage and may be helpful in the metabolic engineering of carotenoid biosynthesis in plants.en
dc.language.isoende
dc.relation.ispartofseriesEXCLI Journal ; Vol. 11, 2012en
dc.subjectBrassica rapa subsp. pekinensisen
dc.subjectcarotenoid biosynthesis genesen
dc.subjectcarotenoidsen
dc.subjectChinese cabbageen
dc.subjectβ-caroteneen
dc.subject.ddc610-
dc.titleAnalysis of carotenoid accumulation and expression of carotenoid biosynthesis genes in different organs of Chinese cabbage (Brassica rapa subsp. pekinensis)en
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access-
eldorado.dnb.zdberstkatid2132560-1-
Appears in Collections:Original Articles

Files in This Item:
File Description SizeFormat 
Park_22082012_proof.pdfDNB393.53 kBAdobe PDFView/Open


This item is protected by original copyright



This item is protected by original copyright rightsstatements.org