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DC Element | Wert | Sprache |
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dc.contributor.author | Tantimongcolwat, Tanawut | - |
dc.contributor.author | Isarankura-Na-Ayudhya, Chartchalerm | - |
dc.contributor.author | Srisarin, Apapan | - |
dc.contributor.author | Galla, Hans-Joachim | - |
dc.contributor.author | Prachayasittikul, Virapong | - |
dc.date.accessioned | 2014-07-24T09:03:13Z | - |
dc.date.available | 2014-07-24T09:03:13Z | - |
dc.date.issued | 2014-04-07 | - |
dc.identifier.issn | 1611-2156 | - |
dc.identifier.uri | http://hdl.handle.net/2003/33528 | - |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-15513 | - |
dc.description.abstract | A simple, inexpensive and field applicable metal determination system would be a powerful tool for the efficient control of metal ion contamination in various sources e.g. drinking-water, water reservoir and waste discharges. In this study, we developed a cell-based metal sensor for specific and real-time detection of copper ions. E. coli expressing metal-sensing green fluorescent protein (designated as TG1/(CG)6GFP and TG1/H6CdBP4GFP) were constructed and served as a metal analytical system. Copper ions were found to exert a fluorescence quenching effect, while zinc and cadmium ions caused minor fluorescence enhancement in the engineered bacterial suspension. To construct a user-friendly and reagentless metal detection system, TG1/H6CdBP4GFP and TG1/(CG)6GFP were encapsulated in polyacrylamide hydrogels that were subsequently immobilized on an optical fiber equipped with a fluorescence detection module. The sensor could be applied to measure metal ions by simply dipping the encapsulated bacteria into a metal solution and monitoring fluorescence changes in real time as a function of the metal concentration in solution. The sensor system demonstrated high specificity toward copper ions. The fluorescence intensities of the encapsulated TG1/(CG)6GFP and TG1/H6CdBP4GFP were quenched by approximately 70 % and 80 % by a high-dose of copper ions (50mM), respectively. The level of fluorescence quenching exhibited a direct correlation with the copper concentration, with a linear correlation coefficient (r) of 0.99. The cell-based metal sensor was able to efficiently monitor copper concentrations ranging between 5 µM and 50 mM, encompassing the maximum allowed copper contamination in drinking water (31.15 µM) established by the WHO. Furthermore, the cell-based metal sensor could undergo prolonged storage for at least 2 weeks without significantly influencing the copper sensitivity. | en |
dc.language.iso | en | - |
dc.relation.ispartofseries | EXCLI Journal ; Vol. 13, 2014 | en |
dc.subject | green fluorescent protein | en |
dc.subject | metal sensor | en |
dc.subject | copper | en |
dc.subject | optical sensor | en |
dc.subject.ddc | 610 | - |
dc.title | Polyacrylamide hydrogel encapsulated E. coli expressing metal-sensing green fluorescent protein as a potential tool for copper ion determination | en |
dc.type | Text | - |
dc.type.publicationtype | article | - |
dcterms.accessRights | open access | - |
eldorado.dnb.zdberstkatid | 2132560-1 | - |
Enthalten in den Sammlungen: | Original Articles |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Prachayasittikul_07042014_proof.pdf | DNB | 812.9 kB | Adobe PDF | Öffnen/Anzeigen |
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