Authors: Agbo, Matthias Onyebuchi
Nworu, Chukwuemeka Sylvester
Okoye, Festus Basden Chiedu
Osadebe, Patience Ogoamaka
Title: Isolation and structure elucidation of polyphenols from Loranthus micranthus Linn. parasitic on Hevea brasiliensis with antiinflammatory property
Language (ISO): en
Abstract: The present study was carried out to evaluate the anti-inflammatory activities of polyphenols isolated from the leaves of mistletoe (Loranthus micranthus Linn.) parasitic on Hevea brasiliensis. The anti-inflammatory properties of the isolated compounds were evaluated on the basis of their ability to inhibit the production of nitric oxide (NO) and tumuor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS) activated RAW 264.7 mouse macrophages. Semipreparative HPLC separation of the ethyl acetate (EtOAc) and butanol (n-BuOH) fractions of the leaves of mistletoe (Loranthus micranthus Linn) parasitic on Hevea brasiliensisled to the isolation of four polyphenols: 3-O-(3,4,5-trimethoxybenzoyl)-(-)-epicatechin (TMECG) (1);(-)-epicatechin-3-O-(3′′-O-methyl)-gallate (ECG3′′Me) (2); rutin (3) and peltatoside (4). Compounds 1-4 were isolated for the first time from this plant while 1 was isolated for the first time in nature. These compounds (1-4) were readily identified by comparison of their spectroscopic data with those reported in the literature. The polyphenols proved to have anti-inflammatory activity as evidenced by the suppression of inducible nitric oxide (iNO) and cytokine (TNF-α) levels in the culture supernatant of lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages. However, the study showed that the quercetin diglycosides showed stronger inhibition of proinflammatory mediators than the epicatechin derivates. These data provide evidence that polyphenolic compounds isolated from the mistletoe parasitic on Hevea brasiliensis may contribute to its anti-inflammatory properties by inhibiting the expression of inducible nitric oxide and proinflammatory cytokines such as tumour necrosis factor-α.
Subject Headings: mistletoe
structural elucidation
inducible nitric oxide
tumour necrosis factor- α (TNF- α )
RAW 264.7 murine macrophages
Issue Date: 2014-08-20
Appears in Collections:Original Articles

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