Authors: | Xiao, Kai-Jun Wang, Wen-Xia Dai, Jia-Li Zhu, Liang |
Title: | Anti-inflammatory effect of selagin-7-O-(6''-O-acetyl-)-ß-D-glycoside isolated from Cancrinia discoidea on lipopolysaccharide-induced mouse macrophage RAW 264.7 cells |
Language (ISO): | en |
Abstract: | Selagin-7-O-(6''-O-acetyl-)-β-D-glycoside, a new flavone glycoside isolated from Cancrinia discoidea, is known to exhibit anti-inflammatory activity in vivo. This study aimed to investigate the protection of this flavone glycoside on inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The effects of selagin-7-O-(6''-O-acetyl-)-β-D-glycoside on inflammatory cytokines and signaling pathways were analyzed by enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and western blot. Results show that selagin-7-O-(6''-O-acetyl-)-β-D-glycoside protected LPS-induced macrophage RAW 264.7 cells from injury. The flavone glycoside markedly inhibited the LPS-induced production of tumor necrosis factor-α, interleukin-1β, and interleukin-6 and increased interleukin-10 release in a concentration-dependent manner. Furthermore, treatment with the flavone glycoside decreased nitric oxide and prostaglandin E2 in LPS-challenged RAW 264.7 cells. These decreases were associated with the down-regulation of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and nuclear factor kappa B (NF-κB) activity. These findings suggest that the anti-inflammatory effects of selagin-7-O-(6''-O-acetyl-)-β-D-glycoside were associated with the adjustment of in flammatory cytokines, and attributed to the down-regulation of NF-κB and consequent suppression of the expression of iNOS and COX-2. |
Subject Headings: | selagin-7-O-(6''-O-acetyl-)- β -D-glycoside LPS macrophage cytokine Nuclear factor- κB |
URI: | http://hdl.handle.net/2003/33735 http://dx.doi.org/10.17877/DE290R-7043 |
Issue Date: | 2014-09-09 |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Zhu_09092014_proof.pdf | DNB | 413.8 kB | Adobe PDF | View/Open |
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