Authors: | Patel, Neeraj K. Khan, Mohd. Shahid Bhutani, Kamlesh K. |
Title: | Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model |
Language (ISO): | en |
Abstract: | Silica gel column chromatography fractionation of the dichloromethane extract (LCD) of Leucas cephalotes (Roth.) Spreng. led to the isolation of five compounds namely β-sitosterol (1) + stigmasterol (2), lupeol (3), oleanolic acid (4) and laballenic acid (5). Also, gas chromatography-mass spectrometry (GC-MS) analysis of sub-fraction (LCD-F1) of this extract showed the presence of eleven (6-16) compounds. In addition to this, 3-5 and LCD-F1 were evaluated for lipopolysachharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in RAW 264.7 and J774A.1 cells. Results directed that 4 and 5 were found to inhibit these mediators at half maximal inhibitory concentration of 17.12 to 57.20 μM while IC50 for LCD-F1 was found to be 15.56 to 31.71 μg/mL. Furthermore, LCD at a dose of 50, 100 and 400 mg/Kg was found to reduce significantly LPS induced tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in female Sprague Dawley (SD) rats. All the results findings evoked that the anti-inflammatory effects of Leucas cephalotes is partially mediated through the suppression of pro-inflammatory mediators and hence can be utilized for the development of anti-inflammatory candidates. |
Subject Headings: | Leucas cephalotes GC-MS anti-inflammatory nitric oxide tumor necrosis factor-α interleukin-1β |
URI: | http://hdl.handle.net/2003/34967 http://dx.doi.org/10.17877/DE290R-17015 |
Issue Date: | 2015-04-10 |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Bhutani_10042015_proof.pdf | DNB | 357.64 kB | Adobe PDF | View/Open |
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