Authors: Ramboer, Eva
Rogiers, Vera
Vanhaecke, Tamara
Vinken, Mathieu
Title: Effects of Trichostatin A on drug uptake transporters in primary rat hepatocyte cultures
Language (ISO): en
Abstract: The present study was set up to investigate the effects of Trichostatin A (TSA), a prototypical epigenetic modifier, on the expression and activity of hepatic drug uptake transporters in primary cultured rat hepatocytes. To this end, the expression of the sinusoidal transporters sodium-dependent taurocholate cotransporting polypeptide (Ntcp) and organic anion transporting polypeptide 4 (Oatp4) was monitored by real-time quantitative reverse transcriptase polymerase chain reaction analysis and immunoblotting. The activity of the uptake transporters was analyzed using radiolabeled substrates and chemical inhibitors. Downregulation of the expression and activity of Oatp4 and Ntcp was observed as a function of the cultivation time and could not be counteracted by TSA. In conclusion, the epigenetic modifier TSA does not seem to exert a positive effect on the expression and activity of the investigated uptake transporters in primary rat hepatocyte cultures.
Subject Headings: epigenetics
Trichostatin A
drug transporters
primary hepatocyte culture
URI: http://hdl.handle.net/2003/35001
http://dx.doi.org/10.17877/DE290R-17049
Issue Date: 2015-05-05
Appears in Collections:Original Articles

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