Authors: Bagga, Paramdeep
Ansari, Tarique Mahmood
Siddiqui, Hefazat Hussain
Syed, Asad
Bahkali, Ali H.
Rahman, Md. Azizur
Khan, Mohd. Sajid
Title: Bromelain capped gold nanoparticles as the novel drug delivery carriers to aggrandize effect of the antibiotic levofloxacin
Language (ISO): en
Abstract: To develop bromelain capped gold nanoparticles (BRN capped Au-NPs) as the effective drug delivery carriers of the antibiotic levofloxacin (LvN) and evaluate antibacterial potential of its bioconjugated form compared to pure LvN. BRN capped Au-NPs were synthesized by in vitro method and bioconjugated to LvN using 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide as activator to form Au-BRN-LvN-NPs. These were characterized for mean particle size by dynamic light scattering analysis, zeta potential by Zetasizer nanosystem analysis and transmission electron microscopy (TEM) on carbon coated TEM copper grids by TEM respectively. Drug loading efficiency of LvN was calculated using UV-visible spectroscopy by standard curve of pure LvN. Antibacterial efficacy of Au-BRN-LvN-NPs and pure LvN was determined by evaluating minimum inhibitory concentration (MIC) against Staphylococcus aureus and Eschereschia coli.Two peaks were observed in Au-BRN-LvNNPs spectrum one at 307 nm and other at 526 nm while one peak in BRN capped Au-NPs at 522 nm during UV spectroscopy suggesting red shift. The drug loading efficiency of LvN was found to be 84.8 ± 2.41 %. The diameter of Au-BRN-LvN-NPs and BRN capped Au-NPs were found to be (58.65 ± 2 nm, 38.11 ± 2 nm), zeta potential (-9.01 mV, -13.8 mV) and surface morphology (~13.2 nm, 11.4 nm) respectively. The MICs against S. aureus and E. coli were found to be (0.128 μg/mL, 1.10 μg/mL) for Au-BRN-LvN-NPs and (0.547 μg/mL, 1.96μg/mL) for pure LvN. The results suggested that BRN capped Au-NPs can be used as effective drug delivery carriers of the antibiotic LvN. The Au-BRN-LvN-NPs exhibited enhanced antibacterial activity compared to pure LvN alone.
Subject Headings: antibacterial activity
bromelain
gold nanopart icles
levofloxacin
novel drug delivery carriers
URI: http://hdl.handle.net/2003/35854
http://dx.doi.org/10.17877/DE290R-17878
Issue Date: 2016-12-06
Rights link: https://creativecommons.org/licenses/by/4.0/
Appears in Collections:Original Articles

Files in This Item:
File Description SizeFormat 
Khan_06122016_proof.pdfDNB1.07 MBAdobe PDFView/Open


This item is protected by original copyright



All resources in the repository are protected by copyright.