Authors: Upadhyay, Tarun K.
Fatima, Nida
Sharma, Deepak
Saravanakumar, V.
Sharma, Rolee
Title: Preparation and characterization of beta-glucan particles containing a payload of nanoembedded rifabutin for enhanced targeted delivery to macrophages
Language (ISO): en
Abstract: β-glucan particles (GP) are polymeric carbohydrates, mainly found as components of cell wall fungi, yeast, bacteria and also in cereals such as barley and oat, and have been recently shown to have application in macrophagetargeted drug delivery. The aim of this study was to prepare and characterize GP containing a large payload of Rifabutin (RB), an anti-tuberculosis drug effective against MDR-TB at lower MIC than Rifampicin. GP were prepared from yeast cells by acidic and alkaline extraction were either spray dried or lyophilized, prior to RB loading and alginate sealing. The FTIR and 13C-NMR spectra of the GP confirmed a β-(1→3) linked glucan structure, with a triple-helical conformation. The spray dried GP exhibited better characteristics in terms of uniformity, size range (2.9 to 6.1 μm) and more than 75 % particles were below 3.5 μm. The RP-HPLC analysis of spray dried GP revealed drug entrapment and drug loading up to 81.46 ± 4.9 % and ~40.5 ± 1.9 %, respectively, as compared to those dried by lyophilization. Electron microscopy showed nearly spherical and porous nature of GP, and the presence of drug ‘nanoprecipitates’ filling the pore spaces. The formulation showed adequate thermal stability for pharmaceutical application. The particles were readily phagocytosed by macrophage(s) within 5 min of exposure. Drug release occurred in a sustained manner via diffusion, as the release kinetics best fit for drug release was obtained using Higuchi’s equation. Thus, the spray dried GP-based-formulation technology holds promise for enhanced targeted delivery of anti-TB drug(s) to macrophage within a therapeutic window for the clearance of intracellular bacteria.
Subject Headings: glucan particles
targeted drug delivery
spray drying
macrophage
rifabutin
phagocytosis
URI: http://hdl.handle.net/2003/36066
http://dx.doi.org/10.17877/DE290R-18082
Issue Date: 2017-03-07
Appears in Collections:Original Articles

Files in This Item:
File Description SizeFormat 
Sharma_07032017_proof.pdfDNB1.06 MBAdobe PDFView/Open


This item is protected by original copyright



All resources in the repository are protected by copyright.