Authors: Nouri Nojadeh, Jafar
Hashemzadeh, Shahriar
Samadi Kafil, Hossein
Behrouz Sharif, Shahin
Eftekharsadat, Amirtaher
Ghasemnejad, Tohid
Ghojazadeh, Mortaza
Sakhinia, Ebrahim
Title: Evaluation of microsatellite instability in tumor and tumor marginal samples of sporadic colorectal cancer using mononucleotide markers
Language (ISO): en
Abstract: Microsatellite instability (MSI) is a unique molecular alteration that is due to a defective DNA mismatch repair (MMR) system. Approximately, 15-20 % of sporadic colorectal cancers (CRC) display MSI. Determination of MSI status in CRC has prognostic and predictive implications. Additionally, detecting MSI is used diagnostically for tumor detection and classification. The present study analyzed a panel of five mononucleotide markers, BAT- 25, BAT-26, NR-21, NR-22 and NR-27, amplified in a single multiplex PCR reaction to evaluate MSI status in CRC patients. Genomic DNA from 50 CRC and paired adjacent normal tissues was used for PCR-based MSI analysis. Our finding showed microsatellite instability in 36 % of specimens. Instability with differences in allele lengths was observed in the tumoral DNA compared to the tumor-free margin DNA sample. The frequency of instability in NR-21, BAT-26 and BAT-25 markers were more than others; their frequency were 35.48 %, 29.03 %, and 22.58 %, respectively. In conclusion, the NR-21, BAT-26, and BAT-25 were the most useful markers for discriminating cancer tissue from normal, therefore these markers have demonstrated promising potential for determining MSI status in patients with sporadic colorectal cancer.
Subject Headings: CRC
DNA MMR system
Issue Date: 2018-09-24
Rights link:
Appears in Collections:Original Articles

Files in This Item:
File Description SizeFormat 
Sakhinia_24092018_proof.pdfDNB175.67 kBAdobe PDFView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons