Authors: | Chatupheeraphat, Chawalit Nantasenamat, Chanin Deesrisak, Kamolchanok Roytrakul, Sittiruk Anurathapan, Usanarat Tanyong, Dalina |
Title: | Bioinformatics and experimental studies of anti-leukemic activity from 6-gingerol demonstrate its role in p53 mediated apoptosis pathway |
Language (ISO): | en |
Abstract: | 6-gingerol is a traditional medicine that possesses anti-cancer activity against several types of cancer. However, the mechanism of action still remains unclear. Therefore, this study explored the effects of 6-gingerol on anti-leukemic mechanisms in NB4, MOLT4, and Raji leukemic cell. Results indicated that 6-gingerol inhibited cell proliferation and induced cell apoptosis in these 3 cell lines. Moreover, 6-gingerol was shown to increase the mRNA expression of the caspase family thereby suggesting that 6-gingerol induced apoptosis through the caspase-dependent pathway. To explore the signaling pathway regulating 6-gingerol induced apoptosis, we utilized and integrated the network pharmacology approach together with experimental investigations. Targets of 6-gingerol were identified from ChEMBL and STITCH databases, which were used for constructing the protein-protein interaction (PPI) network. Results from the PPI network indicated that p53 was a key regulator. Moreover, it was found that 6-gingerol could increase the levels of p53 mRNA in all leukemic cell lines. Thus, 6-gingerol has shown to have anti-cancer activity. In addition, p53, BAX and BCL2 could be involved in the apoptosis pathway of these leukemic cells. This study is anticipated to be useful for the development of 6-gingerol as an anti-leukemic drug in the future. |
Subject Headings: | 6-gingerol Leukemia Apoptosis Network pharmacology p53 Bioinformatics |
URI: | http://hdl.handle.net/2003/39874 http://dx.doi.org/10.17877/DE290R-21765 |
Publishers Link: | https://www.excli.de/index.php/excli/article/view/1805 |
Issue Date: | 2020-05-06 |
Rights link: | https://creativecommons.org/licenses/by/4.0/ |
Provenance: | IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund |
Citation: | Chatupheeraphat, C., Nantasenamat, C., Deesrisak, K., Roytrakul, S., Anurathapan, U., & Tanyong, D. (2020). Bioinformatics and experimental studies of anti-leukemic activity from 6-gingerol demonstrate its role in p53 mediated apoptosis pathway. EXCLI Journal, 19, 582-595. https://doi.org/10.17179/excli2019-2008 |
Appears in Collections: | Original Articles 2020 |
Files in This Item:
File | Description | Size | Format | |
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Tanyong_06052020_proof.pdf | DNB | 1.13 MB | Adobe PDF | View/Open |
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