Alteration of the conformational dynamics of a DNA hairpin by α-synuclein in the presence of aqueous two-phase systems

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dc.contributor.authorMukherjee, Sanjib K.
dc.contributor.authorKnop, Jim-Marcel
dc.contributor.authorMöbitz, Simone
dc.contributor.authorWinter, Roland
dc.date.accessioned2021-07-29T07:54:22Z
dc.date.available2021-07-29T07:54:22Z
dc.date.issued2020-05-26
dc.description.abstractThe effect of an amyloidogenic intrinsically disordered protein, α-synuclein, which is associated with Parkinson's disease (PD), on the conformational dynamics of a DNA hairpin (DNA-HP) was studied by employing the single-molecule Förster resonance energy transfer method. The open-to-closed conformational equilibrium of the DNA-HP is drastically affected by binding of monomeric α-synuclein to the loop region of the DNA-HP. Formation of a protein-bound intermediate conformation is fostered in the presence of an aqueous two-phase system mimicking intracellular liquid-liquid phase separation. Using pressure modulation, additional mechanistic information about the binding complex could be retrieved. Hence, in addition to toxic amyloid formation, α-synuclein may alter expression profiles of disease-modifying genes in PD. Furthermore, these findings might also have significant bearings on the understanding of the physiology of organisms thriving at high pressures in the deep sea.en
dc.identifier.urihttp://hdl.handle.net/2003/40342
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-22217
dc.language.isoende
dc.relation.ispartofseriesChemistry - a european journal;Vol. 26. 2020, issue 48, pp 10987-10991
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiophysicsen
dc.subjectDNA hairpinen
dc.subjectHigh pressure chemistryen
dc.subjectsmFRETen
dc.subjecta-synucleinen
dc.subject.ddc540
dc.subject.rswkBiophysikde
dc.subject.rswkHaarnadelschleifede
dc.subject.rswkHochdruckchemiede
dc.subject.rswkFluoreszenz-Resonanz-Energie-Transferde
dc.subject.rswkSynuclein <alpha->de
dc.titleAlteration of the conformational dynamics of a DNA hairpin by α-synuclein in the presence of aqueous two-phase systemsen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationChemistry - a european journal. Vol. 26. 2020, issue 48, pp 10987-10991. Special issue: 8th EuChemS Chemistry Congressde
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/chem.202002119de

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