Neuroprotective mechanism of low-dose sodium nitrite in oxygen-glucose deprivation model of cerebral ischemic stroke in PC12 cells

Simple item page
dc.contributor.authorShakib, Nader
dc.contributor.authorKhadem Ansari, Mohammad Hassan
dc.contributor.authorKarimi, Pouran
dc.contributor.authorRasmi, Yousef
dc.date.accessioned2019-08-16T12:47:35Z
dc.date.available2019-08-16T12:47:35Z
dc.date.issued2019-04-08
dc.description.abstractThe purpose of this study was to clarify the mechanisms of the protective effects of low-dose sodium nitrite (SN) on oxygen and glucose deprivation (OGD)-induced endoplasmic reticulum (ER) stress in PC12 cells. The PC12 cells were exposed to 4 h of OGD and treated with 100 μmol SN. The expression and activity of ER stress markers, including PKR-like endoplasmic reticulum kinase (PERK), transcription factor 6 (ATF6), CCAAT/enhancer binding protein homologous protein (CHOP), as well as caspase-12 and -3, were detected by immunoblotting assay. Fluorescence staining was used to detect the levels of reactive oxygen species (ROS) and Ca2+ release from the ER. Cell viability was also evaluated by MTT assay. It was found that SN significantly inhibited ROS production and Ca2+ release from the ER in OGD-injured PC12 cells. Moreover, ER stress marker expression and cleaved fragments of caspase-3 and -12 in OGD-injured PC12 cells were decreased after SN treatment. These findings were accompanied by a significant increase in cell viability. It seems that SN exerts a neuroprotective effect at least partially through reduction of ROS-mediated ER stress caused by OGD insult.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/38182
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-20161
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 18 2019
dc.subjectSodium nitriteen
dc.subjectOxygen and glucose deprivationen
dc.subjectPC12 cellsen
dc.subjectEndoplasmic reticulum stressen
dc.subjectCa2+ releaseen
dc.subject.ddc610
dc.titleNeuroprotective mechanism of low-dose sodium nitrite in oxygen-glucose deprivation model of cerebral ischemic stroke in PC12 cellsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Ansari_Karimi_08042019_proof.pdf
Size:
485.17 KB
Format:
Adobe Portable Document Format
Description:
DNB
Download
Thumbnail Image
Name:
Ansari_Karimi_08042019_supplementary_data.pdf
Size:
1.46 MB
Format:
Adobe Portable Document Format
Description:
DNB
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Original Articles