Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model
| dc.contributor.author | Patel, Neeraj K. | |
| dc.contributor.author | Khan, Mohd. Shahid | |
| dc.contributor.author | Bhutani, Kamlesh K. | |
| dc.date.accessioned | 2016-05-18T11:29:23Z | |
| dc.date.available | 2016-05-18T11:29:23Z | |
| dc.date.issued | 2015-04-10 | |
| dc.description.abstract | Silica gel column chromatography fractionation of the dichloromethane extract (LCD) of Leucas cephalotes (Roth.) Spreng. led to the isolation of five compounds namely β-sitosterol (1) + stigmasterol (2), lupeol (3), oleanolic acid (4) and laballenic acid (5). Also, gas chromatography-mass spectrometry (GC-MS) analysis of sub-fraction (LCD-F1) of this extract showed the presence of eleven (6-16) compounds. In addition to this, 3-5 and LCD-F1 were evaluated for lipopolysachharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in RAW 264.7 and J774A.1 cells. Results directed that 4 and 5 were found to inhibit these mediators at half maximal inhibitory concentration of 17.12 to 57.20 μM while IC50 for LCD-F1 was found to be 15.56 to 31.71 μg/mL. Furthermore, LCD at a dose of 50, 100 and 400 mg/Kg was found to reduce significantly LPS induced tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in female Sprague Dawley (SD) rats. All the results findings evoked that the anti-inflammatory effects of Leucas cephalotes is partially mediated through the suppression of pro-inflammatory mediators and hence can be utilized for the development of anti-inflammatory candidates. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/34967 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-17015 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 14, 2015 | en |
| dc.subject | Leucas cephalotes | en |
| dc.subject | GC-MS | en |
| dc.subject | anti-inflammatory | en |
| dc.subject | nitric oxide | en |
| dc.subject | tumor necrosis factor-α | en |
| dc.subject | interleukin-1β | en |
| dc.subject.ddc | 610 | |
| dc.title | Investigations on Leucas cephalotes (Roth.) Spreng. for inhibition of LPS-induced pro-inflammatory mediators in murine macrophages and in rat model | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 |